Kudryavtseva M V, Sakuta G A, Skorina A D, Stein G I, Emelyanov A V, Kudryavtsev B N
Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia.
Tissue Cell. 1996 Jun;28(3):279-85. doi: 10.1016/s0040-8166(96)80015-2.
Glycogen content was determined in hepatocytes of different lobule zones of the normal human liver (23 patients without any liver pathology) and the liver of patients with chronic viral B hepatitis (30 patients) and chronic alcohol hepatitis (28 patients). All the patients were males and aged between 17-50 years. Quantitative analysis of the glycogen content in hepatocytes of portal and central lobule zones was carried out in sections of the human liver (material of functional biopsies) stained with PAS-reaction. The measurements were carried out using an image analyser 'Magiscan' which allows combined cytophotometric analysis of a substance in cells and determination of the cell localization in tissue. The results showed significant differences of the glycogen content in different lobule zones in the normal liver and in the liver in chronic viral and alcohol hepatitis. Ratios of glycogen content in hepatocytes of the portal and the central zones of liver lobule were 1.128 +/- 0.004 and 1.061 +/- 0.003 in normal human liver, and liver of patients with chronic viral hepatitis respectively, i.e. the glycogen content in hepatocytes of the portal lobule zone was much higher than in the central lobule zone in the normal liver and in the liver of patients with chronic viral B hepatitis. The ratio in patients with chronic alcohol hepatitis was less than 1.0 (0.930 +/- 0.003), i.e. a significantly higher glycogen content was found in hepatocytes of the central liver lobule zone. Possible mechanisms of this phenomenon are discussed. Thus, the pattern of the glycogen content in hepatocytes of different lobule zones can be used as an indicator of etiology of chronic hepatitis.
测定了正常人类肝脏（23例无任何肝脏病变的患者）以及慢性乙型病毒性肝炎患者（30例）和慢性酒精性肝炎患者（28例）不同肝小叶区肝细胞中的糖原含量。所有患者均为男性，年龄在17至50岁之间。采用过碘酸希夫反应（PAS反应）染色的人肝脏切片（功能性活检材料），对门脉区和中央肝小叶区肝细胞中的糖原含量进行定量分析。测量使用图像分析仪“Magiscan”进行，该仪器可对细胞内物质进行联合细胞光度分析，并确定组织中的细胞定位。结果显示，正常肝脏以及慢性病毒性肝炎和酒精性肝炎肝脏的不同肝小叶区糖原含量存在显著差异。正常人类肝脏和慢性病毒性肝炎患者肝脏中，肝小叶门脉区和中央区肝细胞的糖原含量之比分别为1.128±0.004和1.061±0.003，即正常肝脏和慢性乙型病毒性肝炎患者肝脏中，肝小叶门脉区肝细胞的糖原含量远高于中央肝小叶区。慢性酒精性肝炎患者的该比值小于1.0（0.930±0.003），即中央肝小叶区肝细胞中糖原含量显著更高。本文讨论了这一现象的可能机制。因此，不同肝小叶区肝细胞中糖原含量的模式可作为慢性肝炎病因的一个指标。
