胸膜肺炎放线杆菌中四环素和红霉素耐药性的特征分析

Characterization of tetracycline and erythromycin resistance in Actinobacillus pleuropneumoniae.

作者信息

Wasteson Y, Roe D E, Falk K, Roberts M C

机构信息

Department of Pathobiology, University of Washington, Seattle 98195, USA.

出版信息

Vet Microbiol. 1996 Jan;48(1-2):41-50. doi: 10.1016/0378-1135(95)00130-1.

DOI:10.1016/0378-1135(95)00130-1

PMID:8701576

Abstract

Minimum inhibitory concentrations to tetracycline and erythromycin were determined for nineteen isolates of Actinobacillus pleuropneumoniae of Norwegian origin. The isolates were screened for rRNA methylase determinants (Erm genes) and for the tetracycline resistance Tet B determinant, using oligonucleotide probes, polymerase chain reaction and hybridization. Ten isolates (53%) carried the Erm C determinant, two isolates (10%) carried the Erm A determinant, four isolates (21%) carried both the Erm A and the Erm C determinants, and three isolates (16%) carried none of the Erm determinants examined. Eight isolates (45%) carried the Tet B determinant. Selected isolates were shown to transfer the Erm C and Erm A determinants at a frequency of 10(-7)-10(-9) per recipient cell. This is the first description of A. pleuropneumoniae carrying either Erm A, Erm C or/and Tet B determinants.

摘要

对19株挪威源胸膜肺炎放线杆菌分离株测定了对四环素和红霉素的最低抑菌浓度。使用寡核苷酸探针、聚合酶链反应和杂交技术，对这些分离株进行rRNA甲基化酶决定簇（Erm基因）和四环素抗性Tet B决定簇的筛选。10株分离株（53%）携带Erm C决定簇，2株分离株（10%）携带Erm A决定簇，4株分离株（21%）同时携带Erm A和Erm C决定簇，3株分离株（16%）未携带所检测的任何Erm决定簇。8株分离株（45%）携带Tet B决定簇。选定的分离株显示，每个受体细胞转移Erm C和Erm A决定簇的频率为10^(-7)-10^(-9)。这是首次描述携带Erm A、Erm C或/和Tet B决定簇的胸膜肺炎放线杆菌。