Vai M, Orlandi I, Cavadini P, Alberghina L, Popolo L
Dipartimento di Fisiologia e Biochimica Generali, Università degli Studi di Milano, Italy.
Yeast. 1996 Mar 30;12(4):361-8. doi: 10.1002/(SICI)1097-0061(19960330)12:4%3C361::AID-YEA920%3E3.0.CO;2-T.
The GGP1/GAS1/CWH52 gene of Saccharomyces cerevisiae encodes a major exocellular 115 kDa glycoprotein (gp115) anchored to the plasma membrane through a glycosylphosphatidylinositol (GPI). The function of gp115 is still unknown but the analysis of null mutants suggests a possible role in the control of morphogenesis. PHR1 gene isolated from Candida alibicans is homologous to the GGP1 gene. In this report we have analysed the ability of PHR1 to complement a ggp1 delta mutation in S. cerevisiae. The expression of PHR1 controlled by its natural promoter or by the GGP1 promoter has been studied. In both cases we have observed a complete complementation of the mutant phenotype. Moreover, immunological analysis has revealed that PHR1 in budding yeast gives rise to a 75-80 kDa protein anchored to the membrane through a GPI, indicating that the signal for GPI attachment present in the C. albicans gene product is functional in S. cerevisiae.
酿酒酵母的GGP1/GAS1/CWH52基因编码一种主要的细胞外115 kDa糖蛋白（gp115），该蛋白通过糖基磷脂酰肌醇（GPI）锚定在质膜上。gp115的功能尚不清楚，但对缺失突变体的分析表明其在形态发生控制中可能发挥作用。从白色念珠菌中分离出的PHR1基因与GGP1基因同源。在本报告中，我们分析了PHR1互补酿酒酵母ggp1δ突变的能力。研究了由其天然启动子或GGP1启动子控制的PHR1的表达。在这两种情况下，我们都观察到突变表型的完全互补。此外，免疫分析表明，出芽酵母中的PHR1产生一种75 - 80 kDa的蛋白，该蛋白通过GPI锚定在膜上，这表明白色念珠菌基因产物中存在的GPI附着信号在酿酒酵母中具有功能。
