Steuer A F, Hentosh P M, Diamond L, Ting R C
Cancer Res. 1977 Jun;37(6):1864-7.
Normal and transformed rat liver epithelial cell lines exhibited differences in the ability to survive in the aggregate form. Normal rat liver epithelial cells in the aggregate form underwent a rapid decline in the number of viable cells, while counterpart transformed epithelial cells exhibited an ability to survive and proliferate in the aggregate form. This survival ability was found to correlate with colony formation in soft agar and tumorigenicity in nude mice. Cell survival in the aggregate form could possibly serve as a criterion for in vitro transformation of epithelial cells derived from rat liver.
正常和转化的大鼠肝上皮细胞系在以聚集形式存活的能力上表现出差异。聚集形式的正常大鼠肝上皮细胞活细胞数量迅速下降，而对应的转化上皮细胞则表现出以聚集形式存活和增殖的能力。发现这种存活能力与软琼脂中的集落形成以及裸鼠中的致瘤性相关。聚集形式的细胞存活可能作为大鼠肝来源上皮细胞体外转化的一个标准。
