Zhou D, Kalaitzís P, Mattoo A K, Tucker M L
Plant Molecular Biology Laboratory, USDA/ARS, Beltsville, MD 20705, USA.
Plant Mol Biol. 1996 Mar;30(6):1331-8. doi: 10.1007/BF00019564.
Dominant mutations in the Arabidopsis ETR1 gene block the ethylene signal transduction pathway. The ETR1 gene has been cloned and sequenced. Using the ETR1 cDNA as a probe, we identified a cDNA homologue (eTAE1) from tomato. eTAE1 contains an open reading frame encoding a polypeptide of 754 amino acid residues. The nucleic acid sequence for the coding sequence in eTAE1 is 74% identical to that for ETR1, and the deduced amino acid sequence is 81% identical and 90% similar. Genomic Southern blot analysis indicates that three or more ETR1 homologues exist in tomato. RNA blots show that eTAE1 mRNA is constitutively expressed in all the tissues examined, and its accumulation in leaf abscission zones was unaffected by ethylene, silver ions (an inhibitor of ethylene action) or auxin.
拟南芥ETR1基因中的显性突变阻断了乙烯信号转导途径。ETR1基因已被克隆和测序。利用ETR1 cDNA作为探针，我们从番茄中鉴定出一个cDNA同源物（eTAE1）。eTAE1含有一个开放阅读框，编码一个由754个氨基酸残基组成的多肽。eTAE1中编码序列的核酸序列与ETR1的核酸序列有74%的同一性，推导的氨基酸序列有81%的同一性和90%的相似性。基因组Southern杂交分析表明番茄中存在三个或更多的ETR1同源物。RNA印迹显示eTAE1 mRNA在所有检测的组织中组成性表达，其在叶片脱落区的积累不受乙烯、银离子（乙烯作用抑制剂）或生长素的影响。
