Bisson Melanie M A, Kessenbrock Mareike, Müller Lena, Hofmann Alexander, Schmitz Florian, Cristescu Simona M, Groth Georg
Biochemical Plant Physiology, Heinrich-Heine-University Düsseldorf, D-40204 Düsseldorf, Germany.
Department of Molecular and Laser Physics, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
Sci Rep. 2016 Aug 1;6:30634. doi: 10.1038/srep30634.
The plant hormone ethylene is involved in the regulation of several processes with high importance for agricultural applications, e.g. ripening, aging and senescence. Previous work in our group has identified a small peptide (NOP-1) derived from the nuclear localization signal of the Arabidopsis ethylene regulator ETHYLENE INSENSITIVE-2 (EIN2) C-terminal part as efficient inhibitor of ethylene responses. Here, we show that NOP-1 is also able to efficiently disrupt EIN2-ETR1 complex formation in tomato, indicating that the NOP-1 inhibition mode is conserved across plant species. Surface application of NOP-1 on green tomato fruits delays ripening similar to known inhibitors of ethylene perception (MCP) and ethylene biosynthesis (AVG). Fruits treated with NOP-1 showed similar ethylene production as untreated controls underlining that NOP-1 blocks ethylene signaling by targeting an essential interaction in this pathway, while having no effect on ethylene biosynthesis.
植物激素乙烯参与调控多个对农业应用至关重要的过程，例如成熟、老化和衰老。我们团队之前的工作已鉴定出一种源自拟南芥乙烯调节因子乙烯不敏感2（EIN2）C末端部分核定位信号的小肽（NOP-1），它是乙烯反应的有效抑制剂。在此，我们表明NOP-1也能够有效破坏番茄中EIN2-ETR1复合物的形成，这表明NOP-1的抑制模式在不同植物物种中是保守的。将NOP-1表面施用于绿色番茄果实上可延缓成熟，这与已知的乙烯感知抑制剂（MCP）和乙烯生物合成抑制剂（AVG）类似。用NOP-1处理的果实与未处理的对照相比，乙烯产量相似，这突出表明NOP-1通过靶向该途径中的关键相互作用来阻断乙烯信号传导，而对乙烯生物合成没有影响。
