Rahman M, Holme T
Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden.
J Med Microbiol. 1996 May;44(5):348-54. doi: 10.1099/00222615-44-5-348.
Antibodies against the serotype determinant epitopes of Moraxella catarrhalis lipopolysaccharides (LPS) were demonstrated in sera from rabbits immunised with whole bacterial cells. Purified LPS preparations from eight strains of M. catarrhalis were used as antigens in enzyme-linked immunosorbent assays (ELISA) and immunoblotting. The serotype specificity of the antibodies was shown by neutralisation with LPS and with purified polysaccharide obtained from LPS prepared from strains belonging to different serotypes. In immunoblots, antisera against the A and B LPS serotypes reacted only with LPS of its own type, confirming the presence of type-specific antibodies. A weak band was observed with type A LPS and antibody to type C, indicating cross-reactivity between the A and C serotypes. This cross-reaction can be explained on the basis of the known chemical structure of the LPS of the different serotypes. After heterologous absorption of sera, bands were obtained with homologous LPS antigen. These results suggest that the predominant antibody response in rabbits to LPS from M. catarrhalis is serotype-specific, unlike that previously observed in infected human patients.
在用全菌细胞免疫的兔血清中，检测到了针对卡他莫拉菌脂多糖（LPS）血清型决定簇表位的抗体。从8株卡他莫拉菌中纯化的LPS制剂用作酶联免疫吸附测定（ELISA）和免疫印迹的抗原。通过用LPS以及从属于不同血清型菌株制备的LPS中获得的纯化多糖进行中和，显示了抗体的血清型特异性。在免疫印迹中，针对A和B LPS血清型的抗血清仅与自身类型的LPS反应，证实了型特异性抗体的存在。观察到A型LPS与C型抗体出现一条弱带，表明A和C血清型之间存在交叉反应。这种交叉反应可以根据不同血清型LPS的已知化学结构来解释。血清进行异源吸收后，用同源LPS抗原获得了条带。这些结果表明，与之前在受感染人类患者中观察到的情况不同，兔对卡他莫拉菌LPS的主要抗体反应是血清型特异性的。
