Harada Daisuke, Takada Chie, Tsukumo Yukihito, Takaba Katsumi, Manabe Haruhiko
Department of Allergy Research, Kyowa Hakko Kogyo Co., Ltd., Sunto-gun, Sizuoka 411-8731, Japan.
J Dermatol Sci. 2005 Mar;37(3):159-67. doi: 10.1016/j.jdermsci.2004.11.007. Epub 2004 Dec 31.
The model of chronic dermatitis caused by repeated application of hapten is frequently used as a tool for assessment of the efficacy of a compound or the elucidation of chronic dermatitis.
The purpose of this study is to provide more detailed analysis of the model of chronic dermatitis caused by repeated application of 2,4,6-trinitro-1-chlorobenzene (TNCB).
BALB/c mice were sensitized with TNCB on day -7 to the ear, and then TNCB was repeatedly applied to the same ear three times per week, through days 0-21.
The repeated application of TNCB induced an increase of ear thickness, and a relatively steep increment of ear thickness from days 7 to 9 was observed. This increment reached almost a plateau at day 9. The peaks of ear swelling on days 7, 14 and 21 were approximately two times higher than that on day 0. Regarding cytokines in the ear, the highest production of IL-1 beta, 4, 6 and 18 were observed on day 7. The peak production of IL-1 beta, 4 and 6 on day 7 was found within 24 h after the challenge, while that of IL-18 was found at 0 h. The inflammatory cell infiltration into epidermis and dermis was observed and increased in day 7, then reached almost a plateau on day 9.
The indices such as thickness, swelling and inflammatory cell infiltration in the lesional skin was increased and maintained by repeated application, however, the protein levels of some cytokines were not always consistent with the reactions.
通过反复涂抹半抗原引发慢性皮炎的模型常被用作评估化合物疗效或阐明慢性皮炎的工具。
本研究旨在对反复涂抹2,4,6-三硝基-1-氯苯（TNCB）引发慢性皮炎的模型进行更详细的分析。
在第-7天对BALB/c小鼠耳部用TNCB进行致敏，然后从第0天至21天每周3次反复将TNCB涂抹于同一耳部。
反复涂抹TNCB导致耳部厚度增加，在第7天至第9天观察到耳部厚度有相对急剧的增加。这种增加在第9天几乎达到平台期。第7天、14天和21天耳部肿胀的峰值比第0天大约高两倍。关于耳部细胞因子，在第7天观察到IL-1β、4、6和18的产生量最高。第7天IL-1β、4和6的产生峰值在激发后24小时内出现，而IL-18的峰值在0小时出现。观察到炎症细胞浸润至表皮和真皮，并在第7天增加，然后在第9天几乎达到平台期。
通过反复涂抹，皮损皮肤的厚度、肿胀和炎症细胞浸润等指标增加并维持，但一些细胞因子的蛋白水平并不总是与反应一致。
