Hidaka K, Ashizawa N, Endoh H, Watanabe M, Fukumoto S
Department of Internal Medicine II, Shimane Medical University, Izumo, Japan.
Int J Pancreatol. 1995 Dec;18(3):207-13. doi: 10.1007/BF02784943.
We observed the cilia in pancreatic ducts (intraductal cilia) with scanning and transmission electron microscopy (SEM and TEM), using male WBN/Kob rats (W/K), which are the spontaneously developed chronic pancreatitis models with stasis of pancreatic juice, and male Wistar rats as control. By SEM observations, the lengths of cilia in interlobular ducts of 18-mo-old W/K were demonstrated to elongate markedly. By TEM observations in the controls, cross-sections of the intraductal cilia were demonstrated to present various numbers of microtubules (those with seven, eight, or nine microtubules accounted for 83.3% of all). There was no significant difference between W/K and controls in the number of microtubules in the cross-sections of intraductal cilia: the intraductal cilium core was provided with nine microtubules, which were different from the number of microtubules encountered within the cilium core of other ciliated cell (i.e., bronchial epithelium, and so forth), and their number in the cross-section of intraductal cilium decreased at a distal portion. Though some of their cross-sections revealed deformities of ciliary membranes and disarrangements of microtubular complex, there was no significant difference in their incidence between both rats. These findings suggest that the intraductal cilia have different functions from the ciliated cells' cilia, and W/K has the elongated intraductal cilia without internal structural change.
我们使用雄性WBN/Kob大鼠（W/K）和雄性Wistar大鼠作为对照，通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察胰腺导管中的纤毛（导管内纤毛）。WBN/Kob大鼠是自发性发展的慢性胰腺炎模型，伴有胰液淤滞。通过SEM观察发现，18月龄W/K大鼠小叶间导管中的纤毛长度明显延长。通过对对照组的TEM观察发现，导管内纤毛的横切面显示出不同数量的微管（有七根、八根或九根微管的占总数的83.3%）。W/K大鼠和对照组导管内纤毛横切面的微管数量没有显著差异：导管内纤毛核心有九根微管，这与其他纤毛细胞（如支气管上皮等）纤毛核心中的微管数量不同，且导管内纤毛横切面的微管数量在远端部分减少。尽管它们的一些横切面显示出纤毛膜的畸形和微管复合体的排列紊乱，但两种大鼠之间的发生率没有显著差异。这些发现表明，导管内纤毛具有与纤毛细胞的纤毛不同的功能，并且W/K大鼠的导管内纤毛伸长但内部结构没有变化。
