Frasca P, Harper R A, Katz J L
Acta Anat (Basel). 1977;98(1):1-13. doi: 10.1159/000144772.
Scanning electron microscopy studies of decalcified human secondary osteons were conducted to determine correlations in fiber orientations in these osteons with their appearance under crossed polaroids. Using a new sample preparation technique, osteons were prepared to expose many of their lamellar interfaces. By studying many such samples, it was concluded that osteons which appear 'dark' under crossed polaroids contain fibers with orientations having little or no transverse component, whereas 'bright' osteons possess fiber orientations with transverse and longitudinal components. 'Intermediate' osteons have been found to contain fiber orientations richer in longitudinal component than in transverse.
对脱钙的人类次级骨单位进行扫描电子显微镜研究，以确定这些骨单位中纤维方向与其在正交偏光镜下外观之间的相关性。采用一种新的样品制备技术，制备骨单位以暴露其许多板层界面。通过研究许多这样的样品，得出的结论是，在正交偏光镜下呈现“暗”色的骨单位含有纤维，其方向几乎没有或没有横向分量，而“亮”骨单位的纤维方向具有横向和纵向分量。已发现“中间”骨单位所含纤维方向的纵向分量比横向分量更丰富。
