Sagawa K, Hagiwara S, Katagiri K, Sakata M, Kawano H, Higashitani T
Department of Transfusion Medicine, Kurume University School of Medicine, Japan.
Kurume Med J. 1996;43(1):25-31. doi: 10.2739/kurumemedj.43.25.
Two murine monoclonal antibodies (mAb) designated as NU-T1 and NU-T2 were generated to identify the novel antigens expressed on human T cells. NU-T1 reacted with peripheral blood T cells, but not with granulocytes or monocytes, whereas NU-T2 were not reactive with peripheral blood lymphocytes, granulocytes or monocytes. Both NU-T1 and NU-T2 reacted with thymocytes. The incidence of NU-T1 or NU-T2 reactivity with the leukemic cells from patients with various types of leukemia was as follows: 12/12 or 4/12 in acute lymphocytic leukemia (ALL) of T cell type, 6/6 or 0/7 in adult T cell leukemia, 0/6 or 0/19 in ALL of non-T non-B cell type, and 0/8 or 0/8 in acute myelocytic leukemia, respectively. The incidence of NU-T1 and NU-T2 reactivity with human leukemic T cell lines were 4/10 and 5/10, respectively. In contrast, neither NU-T1 nor NU-T2 reacted with B cell lines, non-T non-B cell lines, or myeloid cell lines tested. These results indicate that NU-T1 and NU-T2 recognize two distinct differentiation antigens expressed on the cells in the T cell lineage. These mAb would be useful reagents to study the function of human T cells and the immunophenotyping of human T cell malignancies.
产生了两种命名为NU-T1和NU-T2的鼠单克隆抗体（mAb），以鉴定人T细胞上表达的新型抗原。NU-T1与外周血T细胞反应，但不与粒细胞或单核细胞反应，而NU-T2与外周血淋巴细胞、粒细胞或单核细胞无反应。NU-T1和NU-T2均与胸腺细胞反应。NU-T1或NU-T2与各类白血病患者白血病细胞的反应发生率如下：T细胞型急性淋巴细胞白血病（ALL）中分别为12/12或4/12，成人T细胞白血病中为6/6或0/7，非T非B细胞型ALL中为0/6或0/19，急性髓细胞白血病中为0/8或0/8。NU-T1和NU-T2与人白血病T细胞系的反应发生率分别为4/10和5/10。相反，NU-T1和NU-T2均不与所测试的B细胞系、非T非B细胞系或髓细胞系反应。这些结果表明，NU-T1和NU-T2识别T细胞谱系细胞上表达的两种不同的分化抗原。这些单克隆抗体将是研究人T细胞功能和人T细胞恶性肿瘤免疫表型分析的有用试剂。
