Ohsugi T, Kiuchi Y, Shimoda K, Oguri S, Maejima K
Laboratory Animal Center, Keio University School of Medicine, Tokyo, Japan.
Lab Anim. 1996 Jan;30(1):46-50. doi: 10.1258/002367796780744956.
Host defence mechanisms associated with the inhibition of translocation of bacteria from the gastrointestinal (GI) tract were investigated in SCID and beige mice after decontamination with oral antibiotics and colonization with Escherichia coli C25. SCID mice, which have impaired T and B cell function, tended to have a greater incidence of bacterial translocation from the GI tract up to 7 days after inoculation compared with controls. However, after 7 days both SCID and controls cleared the E. coli C25 from the liver, spleen, blood and peritoneal cavity. Beige mice, with impaired NK cell and polymorphonuclear leukocyte function, were not able to clear the inoculated bacteria from their liver by 14 days after inoculation although the controls were cleared by 7 days. Numbers of bacteria in the mesenteric lymph nodes (MLN) of beige mice did not decrease significantly by 14 days after inoculation, whereas numbers in SCID mice decreased markedly within 7 days. These results suggest that defence mechanisms other than T and B cell function are important in the inhibition of systemic infection from the GI tract.
在用口服抗生素去污并接种大肠杆菌C25后，对SCID小鼠和米色小鼠中与抑制细菌从胃肠道（GI）易位相关的宿主防御机制进行了研究。T和B细胞功能受损的SCID小鼠在接种后长达7天内，胃肠道细菌易位的发生率往往高于对照组。然而，7天后，SCID小鼠和对照组均从肝脏、脾脏、血液和腹腔中清除了大肠杆菌C25。自然杀伤细胞（NK）和多形核白细胞功能受损的米色小鼠在接种后14天仍无法从肝脏清除接种的细菌，而对照组在7天内就清除了。接种后14天，米色小鼠肠系膜淋巴结（MLN）中的细菌数量没有显著减少，而SCID小鼠中的细菌数量在7天内显著减少。这些结果表明，除T和B细胞功能外的防御机制在抑制胃肠道的全身感染中很重要。
