Kusunoki S, Iwamori M, Chiba A, Hitoshi S, Arita M, Kanazawa I
Department of Neurology, Faculty of Medicine, University of Tokyo, Japan.
Neurology. 1996 Jul;47(1):237-42. doi: 10.1212/wnl.47.1.237.
Serum antibody from some patients with Guillain-Barré syndrome recognized an antigen of a minor component in human brain monosialoganglioside fraction. We purified that antigen, which migrated at a position slightly lower than that of GM1 on a thin-layer chromatogram (TLC), by using Iatrobeads column chromatography and preparative TLC. Structural analyses, including fast atom bombardment mass spectrometry, showed it to be GM1b. An enzyme-linked immunosorbent assay (ELISA) using purified GM1b showed that anti-GM1b antibody was present in 22 of 104 cases tested. No anti-GM1b antibody was present in the sera from control patients with other diseases or from the normal controls. Four sera recognized only GM1b among the 11 ganglioside antigens tested. The other 18 sera had antibodies to other antigens, most of which shared no terminal epitope with GM1b. Eight of nine sera samples with anti-GalNAc-GD1a antibody also had anti-GM1b antibody. Antibody to a minor monosialoganglioside, GM1b, was found to be a useful diagnostic marker for Guillain-Barré syndrome. Further study is needed to determine whether this antibody plays a role in the pathogenetic mechanism of the syndrome.
一些吉兰-巴雷综合征患者的血清抗体识别出人类脑单唾液酸神经节苷脂组分中一种次要成分的抗原。我们使用免疫亲和层析柱色谱法和制备性薄层色谱法纯化了该抗原，在薄层色谱图（TLC）上，其迁移位置略低于GM1。包括快原子轰击质谱法在内的结构分析表明它是GM1b。使用纯化的GM1b进行的酶联免疫吸附测定（ELISA）显示，在104例检测病例中有22例存在抗GM1b抗体。其他疾病对照患者或正常对照者的血清中不存在抗GM1b抗体。在检测的11种神经节苷脂抗原中，有4份血清仅识别GM1b。其他18份血清含有针对其他抗原的抗体，其中大多数与GM1b没有共同的末端表位。9份含有抗GalNAc-GD1a抗体的血清样本中有8份也含有抗GM1b抗体。发现针对一种次要单唾液酸神经节苷脂GM1b的抗体是吉兰-巴雷综合征的一种有用诊断标志物。需要进一步研究以确定该抗体是否在该综合征的发病机制中起作用。
