Ueno H, Kato I, Ishino Y
Biotechnology Research Laboratories, Takara Shuzo Co. Ltd, Shiga, Japan.
Nucleic Acids Res. 1996 Jun 15;24(12):2268-70. doi: 10.1093/nar/24.12.2268.
BalI, a type II restriction-modification (R-M) system from the bacterium, Brevibacterium albidum, recognizes the DNA sequence 5'-TGGCCA-3'. We cloned the genes encoding the BalI restriction endonuclease and methyltransferase and expressed them in Escherichia coli. The two genes were aligned tail-to-tail and their termination codons overlapped. BalI restriction endonuclease and methyltransferase comprise 260 and 280 amino acids, respectively, and have molecular weights of 29 043 and 31 999 Da. The amino acid sequence of BalI methyltransferase is similar to that of other m6A MTases, although it has been categorized as a m5C methyltransferase. A high expression system for the BalI restriction endonuclease was constructed in E. coli for the production of large quantities of enzyme.
BalI是来自白色短杆菌的一种II型限制-修饰（R-M）系统，可识别DNA序列5'-TGGCCA-3'。我们克隆了编码BalI限制性内切核酸酶和甲基转移酶的基因，并在大肠杆菌中进行表达。这两个基因尾对尾排列，它们的终止密码子重叠。BalI限制性内切核酸酶和甲基转移酶分别由260和280个氨基酸组成，分子量分别为29043和31999道尔顿。BalI甲基转移酶的氨基酸序列与其他m6A甲基转移酶相似，尽管它已被归类为m5C甲基转移酶。在大肠杆菌中构建了BalI限制性内切核酸酶的高效表达系统，用于大量生产该酶。
