两种适应在不同细胞中生长的人冠状病毒OC43株S蛋白的结构与功能分析。
Structural and functional analysis of the S proteins of two human coronavirus OC43 strains adapted to growth in different cells.
作者信息
Künkel F, Herrler G
机构信息
Institut für Virologie, Philipps-Universität Marburg, Federal Republic of Germany.
出版信息
Arch Virol. 1996;141(6):1123-31. doi: 10.1007/BF01718615.
Abstract
The receptor-binding activity of strain CU (grown in MDCK I cells) and of strain VA (adapted to Vero cells) of human coronavirus OC43 was analyzed and compared with the binding activity of bovine coronavirus (BCV) and of the OC43 strain provided by the American Type Culture Collection (AT). Results obtained with resialylated erythrocytes indicated that the ability of the viruses to recognize 9-O-acetylated sialic acid in an alpha 2,6-linkage decreased in the following order: AT > CU > BCV > VA. Only minor differences were observed with respect to the alpha 2,3-linkage. The amino acid sequence of the S protein of strain CU and VA was derived from the nucleotide sequence of the cloned gene. Strain VA differed from strain CU in 34 positions, 18 in the S1 and 16 in the S2 subunit.
摘要
分析并比较了人冠状病毒OC43的CU株（在MDCK I细胞中培养）和VA株（适应于Vero细胞）的受体结合活性，并与牛冠状病毒（BCV）以及美国典型培养物保藏中心（AT）提供的OC43株的结合活性进行比较。用唾液酸化红细胞获得的结果表明，病毒识别α2,6-连接的9-O-乙酰化唾液酸的能力按以下顺序降低：AT＞CU＞BCV＞VA。关于α2,3-连接只观察到微小差异。CU株和VA株S蛋白的氨基酸序列源自克隆基因的核苷酸序列。VA株与CU株在34个位置存在差异，其中S1亚基有18个差异，S2亚基有16个差异。
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