Breed D G, Dorrestein J, Vermeulen A N
Department of Parasitology, Intervet International BV, Boxmeer, The Netherlands.
Avian Dis. 1996 Jan-Mar;40(1):37-48.
The changes in peripheral blood leukocyte (PBL) T-cell subsets following Eimeria tenella infection in outbred white leghorn chickens were studied, using a panel of murine monoclonal antibodies specific for the chicken homologues of the mammalian CD3, CD8, and CD4 markers on day-to-day samples of PBLs. Both flow cytometric analysis (FCA) and immunofluorescence microscopy with fixed cells on slides were used as read-out systems. The changes in the composition of the T-cell subsets measured with both techniques were similar. At 8 days post primary infection, a sharp transitory increase in the proportion of CD8-expressing cells was found. With FCA, CD8-expressing cells could be discriminated in CD8(Dim+) and CD8(Bright+) populations, which have not been described before. The proportion of CD4-expressing cells was decreased at days 9-10 after primary infection, which coincided with a less marked decrease in CD3-expressing cells. Such effects were not seen after secondary infection. When PBLs collected at day 8 post primary infection were stimulated in vitro with E. tenella sporozoite antigen, the response was higher than that in uninfected control chickens. The effects we observed coincide with the onset of recovery from primary infection. We speculate that the increase in CD8-expressing PBLs is the result of stimulation and expansion of a specific subset involved in the induction of protective immunity against Eimeria tenella.
利用一组针对哺乳动物CD3、CD8和CD4标记物的鸡同源物的鼠单克隆抗体，对非近亲繁殖的白来航鸡感染柔嫩艾美耳球虫后外周血白细胞（PBL）T细胞亚群的变化进行了研究，样本为每日采集的PBL。流式细胞术分析（FCA）和对载玻片上固定细胞进行免疫荧光显微镜检查均用作读出系统。两种技术测量的T细胞亚群组成变化相似。初次感染后8天，发现表达CD8的细胞比例急剧短暂增加。通过FCA，可以在CD8（Dim+）和CD8（Bright+）群体中区分表达CD8的细胞，这是以前未曾描述过的。初次感染后第9 - 10天，表达CD4的细胞比例下降，同时表达CD3的细胞也有较不明显的下降。二次感染后未观察到此类效应。当用柔嫩艾美耳球虫子孢子抗原体外刺激初次感染后第8天采集的PBL时，其反应高于未感染的对照鸡。我们观察到的效应与初次感染恢复的开始相吻合。我们推测，表达CD8的PBL增加是参与诱导针对柔嫩艾美耳球虫保护性免疫的特定亚群受到刺激和扩增的结果。
