Arroyave C M, Wolbers R, Ellis P P
Am J Ophthalmol. 1979 Apr;87(4):474-9. doi: 10.1016/0002-9394(79)90233-2.
Venous blood samples were obtained from 85 patients before and at three, ten, and 30 minutes after intravenous administration of sodium fluorescein. Whole complement hemolytic activity and plasma histamine were measured. The patients were observed for side effects. Clinical adverse reactions occurred in 18 (21%) of the patients. Changes in complement hemolytic activity occurred in all patients, but probably were the result of binding of fluorescein to complement proteins, or on the membrane of sensitized red blood cells used in the assay. Plasma histamine was increased within the first few minutes after infusion of fluorescein and persisted up to ten minutes. Increased histamine was found in 66% of patients with adverse reactions and in only 15% of patients with no reactions. Histamine may be an important mediator of adverse reactions to fluorescein.
在85名患者静脉注射荧光素钠前及注射后3分钟、10分钟和30分钟采集静脉血样本。测量全补体溶血活性和血浆组胺。观察患者的副作用。18名（21%）患者出现临床不良反应。所有患者的补体溶血活性均发生变化，但这可能是荧光素与补体蛋白结合的结果，或者是与检测中使用的致敏红细胞膜结合的结果。输注荧光素后的最初几分钟内血浆组胺升高，并持续至10分钟。66%有不良反应的患者和仅15%无反应的患者发现组胺升高。组胺可能是荧光素不良反应的重要介质。
