Xu G, Horiike G, Suzuki T, Miyamoto D, Kumihashi H, Suzuki Y
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, Japan.
Biochem Biophys Res Commun. 1996 Jul 25;224(3):815-8. doi: 10.1006/bbrc.1996.1105.
We reported in a previous paper (1. Xu et al, J. Biochem., 115, 202-207, 1994) that every human influenza B virus isolate determined, including B/Lee/40, B/Setagaya/3/56, B/Tokyo/7/66, B/Kagoshima/1/68, B/Kanagawa/3/76, B/Ibaraki/2/85, B/Yamagata/16/88, and B/Bangkok/163/90, restrictively recognizes lacto-series gangliosides containing Neu5Ac alpha 2-6Gal linkage. We found in this study a novel strain, B/Gifu/2/73, that showed remarkable receptor binding specificities to both Neu5Ac alpha 2-3 Gal and Neu5Ac alpha 2-6Gal linkages of lacto-series gangliosides. The amino acid sequence of the hemagglutinin (HA) in strain B/Gifu/2/73 was compared with that of B/Lee/40, B/Ibaraki/2/85, B/Yamagata/16/88, and B/Bangkok/163/90. Substitution of His116, Ala121, Arg141, Pro211, His253, and His271 in the HA1 subunit of strain B/Gifu/2/73 for Asn116, Thr121, Gly141, Gln211, Gln253, and Gln271 in the HA1 of the four strains above was found, indicating that these amino acid changes in the HA1 of B/Gifu/2/73 may play an important role in recognition of Neu5Ac alpha 2-3Gal and Neu5Ac alpha 2-6Gal linkages in the receptor sialo-sugar chains.
我们在之前的一篇论文（1. 徐等人，《生物化学杂志》，115卷，202 - 207页，1994年）中报道，已测定的每一株人乙型流感病毒，包括B/Lee/40、B/Setagaya/3/56、B/Tokyo/7/66、B/Kagoshima/1/68、B/Kanagawa/3/76、B/Ibaraki/2/85、B/Yamagata/16/88和B/Bangkok/163/90，都具有限制性地识别含有Neu5Acα2 - 6Gal连接的乳糖系列神经节苷脂的特性。在本研究中，我们发现了一种新型毒株B/Gifu/2/73，它对乳糖系列神经节苷脂的Neu5Acα2 - 3Gal和Neu5Acα2 - 6Gal连接均表现出显著的受体结合特异性。将毒株B/Gifu/2/73的血凝素（HA）氨基酸序列与B/Lee/40、B/Ibaraki/2/85、B/Yamagata/16/88和B/Bangkok/163/90的进行了比较。结果发现，B/Gifu/2/73的HA1亚基中的His116、Ala121、Arg141、Pro211、His253和His271分别被上述四种毒株HA1中的Asn116、Thr121、Gly141、Gln211、Gln253和Gln271所取代，这表明B/Gifu/2/73的HA1中的这些氨基酸变化可能在受体唾液酸糖链中对Neu5Acα2 - 3Gal和Neu5Acα2 - 6Gal连接的识别中起重要作用。
