Xu G, Suzuki T, Hanagata G, Deya E, Kiso M, Hasegawa A, Suzuki Y
Department of Biochemistry, University of Shizuoka School of Pharmaceutical Science.
J Biochem. 1993 Mar;113(3):304-7. doi: 10.1093/oxfordjournals.jbchem.a124043.
Sialyl-linkage specificity of the sialidase of influenza B viruses isolated in different years from 1940 through 1990 (B/Lee/40,B/Setagaya/3/56,B/Tokyo/7/66,B/Kagoshima/1/68, B/Gifu/2/73, B/Kanagawa/3/76, B/Ibaraki/2/85, B/Yamagata/16/88, and B/Bangkok/163/90) was studied with N-acetylneuraminyl (alpha 2-3)- and (alpha 2-6)-lactoses, GM3 gangliosides containing the same sialyl-oligosaccharide sequences as sialyllactose, and also with type I and type II lacto-series gangliosides carrying Neu5Ac alpha 2-3Gal and NeuAc alpha 2-6Gal linkages as substrates. From an examination of up to nine strains, the sialidases of all viruses preferentially hydrolyze substrates with Neu5Ac alpha 2-3Gal linkage rather than the Neu5Ac alpha 2-6Gal linkage. It was found that the sialidase activity toward Neu5Ac alpha 2-6Gal linkage relative to Neu5Ac alpha 2-3Gal linkage is increased in later strains, whether sialyllactose or ganglioside is used as the substrate. These results suggested that the sialidase of influenza B virus isolates has shown a drift in linkage specificity which correlates with the year of isolation.
研究了1940年至1990年不同年份分离出的乙型流感病毒（B/Lee/40、B/Setagaya/3/56、B/Tokyo/7/66、B/Kagoshima/1/68、B/Gifu/2/73、B/Kanagawa/3/76、B/Ibaraki/2/85、B/Yamagata/16/88和B/Bangkok/163/90）唾液酸酶的唾液酸连接特异性，所用底物包括N-乙酰神经氨酸基（α2-3）-和（α2-6）-乳糖、含有与唾液酸乳糖相同唾液酸寡糖序列的GM3神经节苷脂，以及携带Neu5Acα2-3Gal和NeuAcα2-6Gal连接的I型和II型乳糖系列神经节苷脂。通过对多达九种毒株的检测，所有病毒的唾液酸酶优先水解具有Neu5Acα2-3Gal连接的底物，而非Neu5Acα2-6Gal连接的底物。结果发现，无论以唾液酸乳糖还是神经节苷脂作为底物，后期毒株中相对于Neu5Acα2-3Gal连接，其对Neu5Acα2-6Gal连接的唾液酸酶活性均有所增加。这些结果表明，乙型流感病毒分离株的唾液酸酶在连接特异性方面出现了漂移，且这种漂移与分离年份相关。
