Imai Y, Ibata I, Ito D, Ohsawa K, Kohsaka S
Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan.
Biochem Biophys Res Commun. 1996 Jul 25;224(3):855-62. doi: 10.1006/bbrc.1996.1112.
We successfully isolated a rat cDNA clone encoding a novel EF hand protein with a molecular weight of about 17 kDa and designated this gene iba1 (ionized calcium binding adapter molecule 1). The genomic copy of the iba1 gene was located within a segment of the major histocompatibility complex class III region between the Bat2 and TNF alpha genes. The iba1 gene was shown to be highly expressed in testis and spleen, but weakly expressed in brain, lung, and kidney. Among brain cells, the iba1 gene was specifically expressed in microglia. A screening of hemopoietic cell lines showed that the Iba1 protein was clearly expressed in monoblastic cell lines but only very weakly expressed in myeloid cell lines. Iba1 protein is therefore suggested to act as an adapter molecule, mediating calcium signals that may function in a monocytic lineage including microglia.
我们成功分离出一个编码新型EF手蛋白的大鼠cDNA克隆，该蛋白分子量约为17 kDa，并将此基因命名为iba1（离子化钙结合衔接分子1）。iba1基因的基因组拷贝位于主要组织相容性复合体III类区域中Bat2和TNFα基因之间的一段区域内。iba1基因在睾丸和脾脏中高表达，但在脑、肺和肾中低表达。在脑细胞中，iba1基因在小胶质细胞中特异性表达。对造血细胞系的筛选显示，Iba1蛋白在单核细胞系中明显表达，但在髓系细胞系中仅非常微弱地表达。因此，Iba1蛋白被认为作为一种衔接分子，介导可能在包括小胶质细胞在内的单核细胞谱系中发挥作用的钙信号。
