核膜组分中的DNA旋转酶活性。
DNA swivel enzyme activity in a nuclear membrane fraction.
作者信息
Yoshida S, Ungers G, Rosenberg B H
出版信息
Nucleic Acids Res. 1977 Jan;4(1):223-8. doi: 10.1093/nar/4.1.223.
Abstract
DNA swivel (nicking-rejoining) enzyme activity has been studied in various cell fractions of a human lymphoid cell line. Swivel activity is found only in chromatin and in a nuclear membrane fraction containing DNA and possessing endogenous DNA synthesizing activity. Twenty percent of the total swivel activity and less than one percent of the total DNA are in the membrane fraction. The swivel enzyme is more tightly bound to the membrane fraction than to the chromatin fraction. These observations suggest that the swivel enzyme may be a replication factor, specifically bound to replicating DNA in the membrane fraction.
摘要
已对人淋巴细胞系的各种细胞组分中的DNA旋转酶（切口-重接）活性进行了研究。旋转酶活性仅在染色质以及含有DNA并具有内源性DNA合成活性的核膜组分中被发现。总旋转酶活性的20%以及不到总DNA 1%存在于膜组分中。旋转酶与膜组分的结合比与染色质组分的结合更紧密。这些观察结果表明，旋转酶可能是一种复制因子，特异性地结合于膜组分中正在复制的DNA。
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