Robbie L A, Bennett B, Croll A M, Brown P A, Booth N A
Department of Medicine and Therapeutics, University of Aberdeen, Scotland, UK.
Thromb Haemost. 1996 Jan;75(1):127-33.
The proteins of fibrinolysis have been quantified in human thrombi, to assess the balance between plasminogen activators and their major inhibitor PAI-1. The relative roles of PAI-1 and alpha 2-AP were also examined since we have previously shown that both platelet PAI-1 and plasma alpha 2-AP are important determinants of clot lysis in vitro. Extracts and sections were prepared from human thrombi for quantitative immunoassay and immunohistochemical staining respectively. PAI-1 and alpha 2-AP were present at high concentrations. Levels of t-PA and t-PA-PAI-1 complex were relatively low. Staining confirmed the presence of abundant PAI-1, associated primarily with platelet material within the thrombus and also with fibrin. Staining for alpha 2-AP was also intense and demonstrated strong association with fibrin. The alpha 2-AP concentration was similar to its high plasma concentration, whereas PAI-1 levels were up to 30 times greater than that in circulating blood, suggesting that active recruitment of platelets contributes to the high PAI-1 concentration in thrombi.
对人血栓中的纤溶蛋白进行了定量分析，以评估纤溶酶原激活剂与其主要抑制剂PAI-1之间的平衡。由于我们之前已表明血小板PAI-1和血浆α2-抗纤溶酶（α2-AP）都是体外凝块溶解的重要决定因素，因此还研究了PAI-1和α2-AP的相对作用。分别从人血栓制备提取物和切片，用于定量免疫测定和免疫组织化学染色。PAI-1和α2-AP浓度很高。组织型纤溶酶原激活剂（t-PA）和t-PA-PAI-1复合物的水平相对较低。染色证实存在大量PAI-1，主要与血栓内的血小板物质以及纤维蛋白相关。α2-AP染色也很强烈，并显示与纤维蛋白有很强的关联。α2-AP浓度与其高血浆浓度相似，而PAI-1水平比循环血液中的水平高30倍，这表明血小板的活跃募集导致血栓中PAI-1浓度升高。
