Lall S B, Peshin S S, Seth S D
Department of Pharmacology, All India Institute of Medical Sciences, New Delhi.
Indian J Exp Biol. 1995 Dec;33(12):921-5.
The study was planned to set up and standardize the radioimmunoassay of Ang-II and to validate the procedure in terms of precision, sensitivity, specificity and recovery. The application of the developed assay was studied in normal healthy volunteers and in patients of renovascular hypertension (RVHT) and renal hypertension (RH). Synthetic human Ang-II was coupled to BSA by carbodimide condensation to get the hapten carrier conjugate which was injected in rabbits to raise the antibodies. The titre of 1:250 showed significant binding (56.79%) and was used for the assay. The sensitivity of the assay was 2 pg/ml and cross-reactivity with analogues of Ang-II was minimum. Mean Ang-II levels in normal subjects was 16 +/- 3.6 pg/ml. In patients of RVHT and RH, the peripheral blood Ang-II levels were found to be 876 +/- 8.6 and 108 +/- 2.3 pg/ml respectively. In patients of unilateral RVHT, renal vein Ang-II levels of the affected side were significantly higher than the unaffected side (P < 0.001). The results indicate that unextracted samples can be successfully utilized to estimate Ang-II levels.
本研究旨在建立并标准化血管紧张素II（Ang-II）的放射免疫分析方法，并在精密度、灵敏度、特异性和回收率方面对该方法进行验证。在正常健康志愿者以及肾血管性高血压（RVHT）和肾性高血压（RH）患者中研究了所开发分析方法的应用。通过碳二亚胺缩合将合成的人Ang-II与牛血清白蛋白（BSA）偶联，得到半抗原载体偶联物，将其注射到兔子体内以产生抗体。1:250的效价显示出显著的结合（56.79%），并用于该分析。该分析的灵敏度为2 pg/ml，与Ang-II类似物的交叉反应性最小。正常受试者的平均Ang-II水平为16±3.6 pg/ml。在RVHT和RH患者中，外周血Ang-II水平分别为876±8.6和108±2.3 pg/ml。在单侧RVHT患者中，患侧肾静脉Ang-II水平显著高于未患侧（P<0.001）。结果表明，未提取的样本可成功用于估计Ang-II水平。
