Tierney M L, Takei Y, Hazon N
School of Environmental and Evolutionary Biology, University of St. Andrews, Fife, KY16 8LB, United Kingdom.
Gen Comp Endocrinol. 1998 Sep;111(3):299-305. doi: 10.1006/gcen.1998.7114.
A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn1, Pro3, Ile5)-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially cross-reacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol . ml-1, respectively.
开发了一种同源放射免疫测定法来测定板鳃亚纲动物中血管紧张素II（天冬酰胺1、脯氨酸3、异亮氨酸5）-血管紧张素II的浓度。与板鳃亚纲动物血管紧张素I和其他异源血管紧张素的交叉反应性很高，因此在用Sep-Pak C18柱进行预提取后，通过高效液相色谱法分离所有可能发生交叉反应的血管紧张素。通过将一系列血管紧张素II标准品与连续稀释的板鳃亚纲动物血浆提取物进行平行测定，证明了该测定法对板鳃亚纲动物血管紧张素II测定的有效性。添加到血浆池中的板鳃亚纲动物血管紧张素II的总回收率为75.1±5.2%。通过我们的放射免疫测定法测得的血浆血管紧张素II浓度在适应70%、100%或120%海水的鱼类中分别为139±20.1、109±15.3和119±16.3 fmol·ml-1，相似。
