Hedlund P, Larsson B, Alm P, Andersson K E
Department of Clinical Pharmacology, Lund University Hospital, Sweden.
Acta Physiol Scand. 1995 Dec;155(4):445-55. doi: 10.1111/j.1748-1716.1995.tb09994.x.
Specimens of penile erectile tissue from the corpus cavernosum (CC) and corpus spongiosum (CS) of beagle dogs were investigated with reference to morphological and functional aspects of the nitric oxide (NO) system. Using immunohistochemistry, the smooth muscle bundles of the CC were found to contain a rich innervation, as seen by the pan-neuronal marker, protein gene product 9.5. A large number of nerves also stained positively for acetylcholine esterase. There was a moderate to rich supply of nerves containing NO synthase, which was frequently co-localized with vasoactive intestinal polypeptide. CS preparations from the proximal penis (the spongious bulb) had an innervation similar to that of the CC. However, CS preparations from the distal penis had a distinctly lower density of nerves than the proximal part, and spongious tissue from the glans penis was practically devoid of nerves. In isolated preparations of proximal and distal CC and CS, contracted by noradrenaline, electrical field stimulation (EFS) produced frequency-dependent relaxations. The responses were less pronounced in preparations from the distal penis, particularly in the CS. Pre-treatment with the NO-synthesis inhibitor NG-nitro-L-arginine abolished all relaxations. Carbachol effectively relaxed noradrenaline-contracted strips. The responses were similar in proximal preparations from the CC and CS, and significantly less pronounced in strips from the distal penis. NG-nitro-L-arginine significantly reduced the relaxant effect of carbachol. Preparations of spongious tissue from the glans penis did not react to high potassium solution, addition of noradrenaline, or EFS. The results demonstrate regional differences within the CS, both in innervation and functional responses.
参照一氧化氮（NO）系统的形态学和功能方面，对比格犬海绵体（CC）和尿道海绵体（CS）的阴茎勃起组织标本进行了研究。使用免疫组织化学方法，通过泛神经元标志物蛋白基因产物9.5观察到，CC的平滑肌束含有丰富的神经支配。大量神经对乙酰胆碱酯酶也呈阳性染色。含有一氧化氮合酶的神经供应中等至丰富，且常与血管活性肠肽共定位。阴茎近端（海绵体球）的CS制剂具有与CC相似的神经支配。然而，阴茎远端的CS制剂神经密度明显低于近端，阴茎头的海绵组织实际上没有神经。在由去甲肾上腺素收缩的近端和远端CC及CS的分离制剂中，电场刺激（EFS）产生频率依赖性舒张。远端阴茎制剂中的反应不太明显，尤其是在CS中。用NO合成抑制剂NG-硝基-L-精氨酸预处理可消除所有舒张反应。卡巴胆碱可有效舒张去甲肾上腺素收缩的条带。CC和CS近端制剂中的反应相似，而远端阴茎条带中的反应明显较弱。NG-硝基-L-精氨酸显著降低了卡巴胆碱的舒张作用。阴茎头球海绵体组织制剂对高钾溶液、添加去甲肾上腺素或EFS均无反应。结果表明，CS内无论是神经支配还是功能反应都存在区域差异。
