Katsuda K, Shirahata T, Kida H, Goto H
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
J Vet Med Sci. 1995 Dec;57(6):1023-7. doi: 10.1292/jvms.57.1023.
Three strains of influenza A virus (H3N2) were isolated from pigs in Hokkaido, Japan in 1993. The hemagglutinin (HA) antigen of the three isolates was related to that of recent H3N2 viruses of human origin. The reactivity patterns of two of the isolates (A/sw/Obihiro/1/93 and A/sw/Obihiro/2/93) with monoclonal antibodies to the hemagglutinin of A/Bangkok/1/79 strain were similar to that of the human H3N2 strain isolated in Hokkaido in 1988, while that of the other one (A/sw/Obihiro/3/93) was similar to human H3N2 strains of 1993. In the phylogenetic analysis based on the nucleotide sequences of the HA1 regions, the HA genes of the two isolates were shown to be closely related to those of human H3N2 viruses isolated between 1986 and 1988. The remaining one isolate was shown to be closely related to those of current human H3N2 viruses. We have also found serological evidence that the A/sw/Obihiro/1/93 virus is circulating extensively in Obihiro swine. It is clear from these findings that pigs were infected with the recent H3N2 influenza virus during the human epidemic and that the virus has been maintained in pigs for at least five years.
1993年，从日本北海道的猪身上分离出三株甲型流感病毒（H3N2）。这三株分离株的血凝素（HA）抗原与近期源自人类的H3N2病毒的抗原相关。其中两株分离株（A/sw/带广/1/93和A/sw/带广/2/93）与针对A/曼谷/1/79株血凝素的单克隆抗体的反应模式，与1988年在北海道分离出的人类H3N2株相似，而另一株（A/sw/带广/3/93）则与1993年的人类H3N2株相似。在基于HA1区核苷酸序列的系统发育分析中，两株分离株的HA基因与1986年至1988年间分离出的人类H3N2病毒的基因密切相关。其余一株分离株与当前人类H3N2病毒的基因密切相关。我们还发现血清学证据表明，A/sw/带广/1/93病毒在带广猪群中广泛传播。从这些发现可以清楚地看出，猪在人类疫情期间感染了近期的H3N2流感病毒，并且该病毒已在猪体内持续存在至少五年。
