Koike T, Titani K, Suzuki M, Beppu H, Kuzuya H, Maruta K, Shimpo K, Fujita K
Institute of Pharmacognosy, Fujita Health University, Mie, Japan.
Biochem Biophys Res Commun. 1995 Sep 5;214(1):163-70. doi: 10.1006/bbrc.1995.2270.
The complete amino acid sequence of a mannose-binding lectin purified from the leaf skin of "Kidachi Aloe" (Aloe arborescens Miller var. natalensis Berger) is presented. The 109-residue sequence of the subunit was determined by analysis of peptides of the intact or S-pyridylethylated protein generated by digestion with cyanogen bromide, BNPS-skatole, Achromobacter protease I, or trypsin. The subunit contains an intrachain disulfide bridge. The sequence is highly homologous to that of a mannose-binding lectin from snowdrop bulb.
本文给出了从“木立芦荟”(库拉索芦荟纳塔尔变种)叶皮中纯化得到的一种甘露糖结合凝集素的完整氨基酸序列。通过分析由溴化氰、BNPS-粪臭素、无色杆菌蛋白酶I或胰蛋白酶消化完整蛋白或S-吡啶基乙基化蛋白产生的肽段,确定了该亚基的109个氨基酸残基序列。该亚基含有一个链内二硫键。其序列与来自雪花莲鳞茎的甘露糖结合凝集素的序列高度同源。