Hamdan J S, Ferrari T C
Department of Microbiology, Federal University of Minas Gerais, Brazil.
Mycoses. 1995 Nov-Dec;38(11-12):481-4. doi: 10.1111/j.1439-0507.1995.tb00024.x.
An atypical strain of Paracoccidioides brasiliensis was obtained from a patient with disseminated paracoccidioidomycosis. This isolate, which has been maintained in the laboratory for 4 years by successive subcultures (30-40 days) in Fava Netto medium, produces cerebriform colonies characteristic of the yeast phase of the fungus when left at room temperature. Microscopy shows a predominance of yeasts with multiple buds, side by side with some branching filamentous structures presenting intercalary chlamydospores. The strain does not develop well at 37 degrees C, suggesting heat sensitivity. Storage at 4 degrees C does not change its macro- or microscopic characteristics. These findings support the hypothesis raised by others that the dimorphism of P. brasiliensis may not be exclusively heat dependent. Studies are currently being developed in our laboratory to better characterize this isolate in terms of biochemical peculiarities, degree of virulence and susceptibility/resistance to antifungal drugs.
一株非典型巴西副球孢子菌从一名播散性副球孢子菌病患者中分离获得。该菌株在实验室中通过在法瓦·内托培养基中连续传代培养（30 - 40天）已保存4年，在室温下放置时会产生该真菌酵母相特有的脑形菌落。显微镜检查显示，以带有多个芽的酵母为主，同时伴有一些带有间生厚垣孢子的分支丝状结构。该菌株在37℃下生长不佳，提示对热敏感。在4℃保存不会改变其宏观或微观特征。这些发现支持了其他人提出的假说，即巴西副球孢子菌的双态性可能并非完全依赖于温度。目前我们实验室正在开展研究，以更好地从生化特性、毒力程度以及对抗真菌药物的敏感性/耐药性方面对该分离株进行表征。
