Onyango-Abuje J A, Hughes G, Opicha M, Nginyi K M, Rugutt M K, Wright S H, Harrison L J
National Veterinary Research Centre, Muguga, Kikuyu, Kenya.
Vet Parasitol. 1996 Feb;61(3-4):221-30. doi: 10.1016/0304-4017(95)00840-3.
Sera from calves, either experimentally or naturally infected with Taenia saginata, were screened for an antibody response to T. saginata, and for parasite antigen, by enzyme-linked immunosorbent assays (ELISAs). An antibody response was detected by 3 weeks post infection (p.i.), rose to a peak at 10-12 weeks p.i., and was still in evidence 1 year p.i. Parasite antigen was first detected 4-7 weeks p.i. and persisted until the end of the experiment, over 1 year p.i. In the experimentally infected animals, cattle with 14 or more live cysticerci had detectable levels of parasite antigen in their sera at slaughter, while animals with live cyst burdens ranging from 0 to 4 were negative. Furthermore, levels of circulating antigen were positively correlated with live cysticercus burden in the experimental animals. In naturally infected cattle, 83% (5/6) of those with 30 or more live cysts, and 22% (5/23) of those with 1-29 live cysts, could be detected by the ELISA for parasite antigen, although no significant correlation between antigen level and live cyst burden could be detected. Antibody levels were not found to be associated with cyst burdens in either experimentally or naturally infected cattle. In slaughterhouse cattle, the antigen assay was almost three times as sensitive as meat inspection. However, there was no agreement between cattle found positive at meat inspection and those found positive by the antigen detection ELISA. One possible reason is that the ELISA only detects live cysts, while lesions left by dead cysts are more noticeable at meat inspection. The mouse monoclonal antibody-based antigen detection ELISA is of value for the diagnosis of naturally occurring, viable, T. saginata cysticercosis in live cattle and has an immediate application for field based epidemiological studies designed to determine prevalence.
通过酶联免疫吸附测定（ELISA），对实验性或自然感染牛带绦虫的犊牛血清进行检测，以确定其对牛带绦虫的抗体反应以及寄生虫抗原。感染后3周可检测到抗体反应，在感染后10 - 12周达到峰值，感染1年后仍可检测到。寄生虫抗原在感染后4 - 7周首次被检测到，并持续到实验结束，即感染1年多后。在实验感染的动物中，有14个或更多活囊尾蚴的牛在屠宰时血清中可检测到寄生虫抗原水平，而活囊尾蚴负担为0至4个的动物则为阴性。此外，实验动物中循环抗原水平与活囊尾蚴负担呈正相关。在自然感染的牛中，ELISA检测寄生虫抗原时，有30个或更多活囊尾蚴的牛中有83%（5/6）呈阳性，有1 - 29个活囊尾蚴的牛中有22%（5/23）呈阳性，尽管未检测到抗原水平与活囊尾蚴负担之间存在显著相关性。在实验性或自然感染的牛中，均未发现抗体水平与囊尾蚴负担有关。在屠宰场的牛中，抗原检测的敏感性几乎是肉类检查的三倍。然而，肉类检查中发现阳性的牛与抗原检测ELISA发现阳性的牛之间并无一致性。一个可能的原因是ELISA仅检测活囊尾蚴，而死囊尾蚴留下的病变在肉类检查中更明显。基于小鼠单克隆抗体的抗原检测ELISA对于诊断活牛自然发生的、存活的牛带绦虫囊尾蚴病具有价值，并且可立即应用于旨在确定患病率的现场流行病学研究。
