Bøgh H O, Grønvold J, Maeda G E, Nansen P, Lind P, Ilsøe B, Henricksen S A, Geerts S
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Res Vet Sci. 1996 Jan;60(1):64-8. doi: 10.1016/s0034-5288(96)90134-4.
Two groups of 12 calves were given either a single infection dose of 30,000 Taenia saginata eggs (group A) or trickle infected with 12 x 2500 T saginata eggs (group B). The calves were killed 16, 22 or 54 weeks after infection and the numbers of viable and dead parasites were counted by slicing selected organs and muscles of half of the carcases. The total numbers of cysts and the numbers of viable cysts were higher in group A than in group B. Within the two groups there were no significant differences between the numbers of cysts at the three dates of slaughter. Comparable results were obtained by using an ELISA and a dot blot procedure for the detection of antibody in the serum of the infected calves; an initial increase in response during the first 10 weeks after infection was generally followed by a gradual decline. When using a monoclonal antibody-based ELISA for the detection of circulating antigens, the sera from all the 14 animals which had no viable cysts or only dead cysts at slaughter did not react with the monoclonal antibodies. Calves 122 and 123, which harboured two and 122 viable cysts, respectively, reacted positively during the late phase of the infection to the antigen-detecting ELISA. However, several other animals which harboured between two and 41 viable cysts at slaughter did not react.
两组12头小牛，一组给予单次感染剂量30000个牛带绦虫卵（A组），另一组以12×2500个牛带绦虫卵进行微量感染（B组）。感染后16、22或54周宰杀小牛，通过对半数 carcasses 的选定器官和肌肉切片来计数存活和死亡寄生虫的数量。A组的囊肿总数和存活囊肿数高于B组。在两组内，三个宰杀日期的囊肿数量之间无显著差异。通过酶联免疫吸附测定（ELISA）和斑点印迹法检测感染小牛血清中的抗体，得到了类似结果；感染后前10周反应最初增加，随后通常逐渐下降。当使用基于单克隆抗体的ELISA检测循环抗原时，宰杀时无存活囊肿或仅有死亡囊肿的所有14只动物的血清均不与单克隆抗体发生反应。分别带有2个和122个存活囊肿的小牛122和123在感染后期对抗原检测ELISA呈阳性反应。然而，宰杀时带有2至41个存活囊肿的其他几只动物没有反应。
