Przyklang U, Nölle B, Reinhold-Keller E, Duncker G
Universitäts-Augenklinik, Kiel, Germany.
Graefes Arch Clin Exp Ophthalmol. 1996 Feb;234(2):79-86. doi: 10.1007/BF00695245.
Ocular involvement in patients with Wegener's granulomatosis (WG) can cause progressive loss of vision. The pathophysiological events leading to the WG-specific ocular inflammation are unknown.
A method involving indirect immunofluorescence on bovine and porcine corneoconjunctival frozen sections was used to detect antibodies to corneal and conjunctival epithelium in serum samples from patients suffering from WG with ophthalmic involvement (orbital granuloma or ocular vasculitis, both active and inactive phase). Normal persons and WG patients without ophthalmic involvement served as control groups. Anti-human IgG, IgA and IgM were tested.
Five different immunofluorescence patterns could be distinguished in each species. There were differences in the reactivity with bovine and porcine tissue. Every pattern was caused mainly by human IgG. The antibodies were directed to the cell nuclei or to extranuclear structures. All patterns also occurred frequently in normal sera. No pattern was found that occurred specifically in the serum of patients with WG and ocular inflammation.
The results indicate that this immunofluorescence system does not detect a characteristic antibody profile that could be attributed to the serum of WG patients. Hence the approach lacks clinical usefulness as a diagnostic procedure.
韦格纳肉芽肿（WG）患者眼部受累可导致视力逐渐丧失。导致WG特异性眼部炎症的病理生理事件尚不清楚。
采用一种对牛和猪角膜结膜冰冻切片进行间接免疫荧光的方法，检测患有眼部受累（眼眶肉芽肿或眼部血管炎，包括活动期和非活动期）的WG患者血清样本中针对角膜和结膜上皮的抗体。正常人和无眼部受累的WG患者作为对照组。检测抗人IgG、IgA和IgM。
在每个物种中可区分出五种不同的免疫荧光模式。与牛和猪组织的反应性存在差异。每种模式主要由人IgG引起。抗体针对细胞核或核外结构。所有模式在正常血清中也经常出现。未发现专门出现在患有眼部炎症的WG患者血清中的模式。
结果表明，该免疫荧光系统未检测到可归因于WG患者血清的特征性抗体谱。因此，作为一种诊断程序，该方法缺乏临床实用性。
