Padmakumar R, Banerjee R
Biochemistry Department, Beadle Center, University of Nebraska, Lincoln 68588-0664, USA.
Biofactors. 1995;5(2):83-6.
Methylmalonyl-CoA mutase is a member of the coenzyme B12-dependent family of isomerases and interconverts methylmalonyl-CoA and succinyl-CoA. We have examined the ability of the enzyme to effect a double rearrangement reaction when presented with glutaryl-CoA, a substrate analog with a three-carbon template on which two successive 1,2 migrations can occur. Our results demonstrate that the enzyme converts glutaryl-CoA to both methylsuccinyl-CoA and ethylmalonyl-CoA. To our knowledge, this is the first example of a double rearrangement reaction catalyzed by a coenzyme B12-dependent enzyme.
甲基丙二酰辅酶A变位酶是依赖辅酶B12的异构酶家族成员，可催化甲基丙二酰辅酶A与琥珀酰辅酶A的相互转化。我们研究了该酶在遇到戊二酰辅酶A时进行双重重排反应的能力，戊二酰辅酶A是一种具有三碳模板的底物类似物，在该模板上可发生两次连续的1,2迁移。我们的结果表明，该酶可将戊二酰辅酶A转化为甲基琥珀酰辅酶A和乙基丙二酰辅酶A。据我们所知，这是依赖辅酶B12的酶催化双重重排反应的首个实例。
