Lippert U, Welker P, Krüger-Krasagakes S, Möller A, Henz B M
Department of Dermatology, Humboldt University, Berlin, Germany.
Skin Pharmacol. 1996;9(2):93-8. doi: 10.1159/000211403.
Mast cells are well known effector cells not only in allergic but also in diverse acute and chronic inflammatory diseases. We have shown previously that these cells produce a broad spectrum of cytokines which might contribute to mast cell-dependent pathology. In the present study, we have investigated the influence of four potent glucocorticoids, methylprednisolone-aceponate, methylprednisolone-17-propionate, prednicarbate, and betametasone valerate (10(-5) M-10(-9) M), on the IL-1 beta, IL-3, IL-8, and tumor necrosis factor alpha secretion of the HMC-1 mast cell line as measured by ELISA. All four glucocorticoids caused a comparable dose- and time-dependent inhibition of cytokine release from HMC-1 cells stimulated for 24 h with phorbol 12-myristate 13-acetate 25 ng/ml and calcium ionophore 2 x 10(-7) M. These results shed further light on the mechanisms involved in antiinflammatory effects of glucocorticoids in allergic inflammation.
肥大细胞不仅是过敏性疾病，也是多种急慢性炎症性疾病中广为人知的效应细胞。我们之前已经表明，这些细胞会产生多种细胞因子，这些细胞因子可能导致肥大细胞依赖性病理变化。在本研究中，我们研究了四种强效糖皮质激素，即醋酸甲泼尼龙、17 - 丙酸甲泼尼龙、泼尼松龙和戊酸倍他米松（10⁻⁵M - 10⁻⁹M），对通过酶联免疫吸附测定法（ELISA）测量的HMC - 1肥大细胞系白细胞介素 - 1β（IL - 1β）、白细胞介素 - 3（IL - 3）、白细胞介素 - 8（IL - 8）和肿瘤坏死因子α（TNF - α）分泌的影响。所有四种糖皮质激素对用25 ng/ml佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯和2×10⁻⁷M钙离子载体刺激24小时的HMC - 1细胞的细胞因子释放均产生了类似的剂量和时间依赖性抑制作用。这些结果进一步揭示了糖皮质激素在过敏性炎症中抗炎作用的机制。
