Gatto E M, Carreras M C, Pargament G A, Riobo N A, Reides C, Repetto M, Fernandez Pardal M M, Llesuy S, Poderoso J J
Section of Neurology, Hospital de Clínicas José de San Martín, School of Medicine, University of Buenos Aires, Argentina.
Mov Disord. 1996 May;11(3):261-7. doi: 10.1002/mds.870110308.
We studied nitrogen radical nitric oxide (.NO) release and reactive oxygen species (ROS) production by isolated neutrophils after phorbol myristate acetate (PMA) stimulation in 12 newly diagnosed and nine treated Parkinson's disease (PD) patients and 10 age-matched healthy controls. Neutrophils of both groups of PD patients had an elevated PMA-activated release of .NO [61 and 57%, respectively, higher than that of controls (p < 0.05)]. In contrast, H2O2 release was only significantly increased by 56% in chronically treated patients. In agreement, the maximum rate of luminol-dependent chemiluminescence, which partly represents O2- H2O2- .NO interactions, was increased only in the treated group. When other blood markers of oxidative stress were compared, only erythrocyte catalase activity was decreased in both PD patient series by 33 and 39%, respectively (p < 0.05), whereas plasma antioxidant capacity and erythrocyte superoxide dismutase activity levels were decreased only in treated PD patients. This study suggests that neutrophils express a primary alteration of .NO release in PD patients, whereas H2O2 and oxidative-stress parameters are more probably related to the evolution of PD or to effects of treatment with L-dopa.
我们研究了12例新诊断的和9例接受治疗的帕金森病（PD）患者以及10名年龄匹配的健康对照者在佛波酯肉豆蔻酸酯乙酸酯（PMA）刺激后，分离的中性粒细胞中氮自由基一氧化氮（·NO）的释放及活性氧（ROS）的产生情况。两组PD患者的中性粒细胞在PMA激活后·NO的释放均有所增加[分别比对照组高61%和57%（p < 0.05）]。相比之下，仅在长期接受治疗的患者中，H2O2的释放显著增加了56%。同样，部分代表O2-、H2O2、·NO相互作用的鲁米诺依赖性化学发光的最大速率仅在治疗组中有所增加。当比较其他氧化应激的血液标志物时，两个PD患者系列中仅红细胞过氧化氢酶活性分别降低了33%和39%（p < 0.05），而血浆抗氧化能力和红细胞超氧化物歧化酶活性水平仅在接受治疗的PD患者中降低。这项研究表明，PD患者中性粒细胞中·NO释放存在原发性改变，而H2O2和氧化应激参数更可能与PD的进展或左旋多巴治疗的效果有关。
