Ilic T V, Jovanovic M, Jovicic A, Tomovic M
Department of Neurology, Military Medical Academy, Belgrade, Yugoslavia.
Funct Neurol. 1999 Jul-Sep;14(3):141-7.
In order to determine the baseline state of oxidative stress indices in drug-free patients with Parkinson's disease (PD), we analyzed cerebrospinal fluid (CSF) and peripheral blood samples of 34 subjects in disease stages I-III, according to the Hoehn & Yahr scale, compared with controls. The results showed a highly significant increase in malondialdehyde content in CSF (p < 0.001) in the patients with PD. We also found significant differences in peripheral blood parameters between the two groups: malondialdehyde content was increased in patients with PD (p < 0.05), as was the activity of antioxidative defense enzymes, glutathione reductase, Cu, and Zn-superoxide-dismutase (p < 0.05). The production of a highly reactive oxygen species--superoxide radical (p < 0.05) was also increased. These findings indicated an important role of oxidative stress in PD evolution and progress. The increased levels of reactive oxidative species (malondialdehyde content and superoxide radical production) in peripheral blood, and excessive activity of protective enzymatic systems (glutathione reductase Cu, and Zn-superoxide-dismutase) could indicate an additional systemic reaction related to a chronic oxidative stress state in the brain.
为了确定未服用药物的帕金森病（PD）患者氧化应激指标的基线状态，我们根据Hoehn & Yahr量表，分析了34例处于I - III期疾病阶段的受试者的脑脊液（CSF）和外周血样本，并与对照组进行比较。结果显示，PD患者脑脊液中的丙二醛含量显著升高（p < 0.001）。我们还发现两组在外周血参数上存在显著差异：PD患者的丙二醛含量升高（p < 0.05），抗氧化防御酶、谷胱甘肽还原酶、铜和锌超氧化物歧化酶的活性也升高（p < 0.05）。高活性氧物质——超氧阴离子的产生也增加了（p < 0.05）。这些发现表明氧化应激在PD的发生和发展中起重要作用。外周血中活性氧化物质水平升高（丙二醛含量和超氧阴离子产生）以及保护性酶系统（谷胱甘肽还原酶、铜和锌超氧化物歧化酶）的过度活性可能表明与大脑慢性氧化应激状态相关的额外全身反应。
