Cho K, McFarlane I D
Department of Applied Biology, University of Hull, UK.
J Neurosci Res. 1996 Apr 15;44(2):184-90. doi: 10.1002/(SICI)1097-4547(19960415)44:2<184::AID-JNR11>3.0.CO;2-6.
Three pulse types, A,B, and C, can be recorded from column ring preparations of the sea anemone Calliactis parasitica (Couch). Lucifer Yellow injection confirmed that the recording sites are intracellular in endodermal myoepithelial cells. Type C pulses are spontaneously active depolarizing pulses, arising from a resting potential of around -60mV, and are usually < 5 mV in amplitude. When the cells were voltage-clamped these pulses reversed at around 0 mV membrane potential; therefore, we conclude that they are synaptic potentials. In some cells an increase in frequency of type C pulses accompanied contraction of the circular muscle field. Inward currents in these myoepithelial cells are carried by Ca2+, and although Na(+)-free solutions did not affect inward currents they did eliminate type C pulses, i.e., these synaptic potentials may result from activity of Na(+)-dependent nerve cells.
从寄生海葵（Calliactis parasitica，库奇）的柱环制剂中可记录到三种脉冲类型，即A、B和C。荧光黄注射证实记录位点位于内胚层肌上皮细胞内。C型脉冲是自发活动的去极化脉冲，由约-60mV的静息电位产生，幅度通常<5mV。当细胞进行电压钳制时，这些脉冲在膜电位约0mV时反转；因此，我们得出结论，它们是突触电位。在一些细胞中，C型脉冲频率的增加伴随着环形肌场的收缩。这些肌上皮细胞中的内向电流由Ca2+携带，尽管无钠溶液不影响内向电流，但它们确实消除了C型脉冲，即这些突触电位可能源于钠依赖性神经细胞的活动。
