Kapanadze B I, Brodianskiĭ V M, Baranova A V, Sevat'ianov S Iu, Fedorova N D, Kurskov M M, Kostina M A, Mironov A A, Sineokiĭ S P, Zakhar'ev V M, Grafodatskiĭ A S, Modianov N N, Iankovskiĭ N K
Genetika. 1996 Mar;32(3):331-40.
We characterized two cosmid libraries constructed from flow-sorted chromosome 13 at the Imperial Cancer Research Fund (ICRF), UK (13,000 clones) and Los Alamos National Laboratory (LANL), USA (17,000 clones). After storage for two years, clones showed high viability (95%) and structural stability. EcoR I and Hind III restriction patterns were studied in more than 500 ICRF and 200 LANL cosmids. The average size of inserts was shown to be 35-37 kb in both the libraries. Most cosmids (83% and 93% of ICRF and LANL libraries, respectively) exceed the lower size limit of DNA fragments that can be packaged and represent a good source for physical mapping of chromosome 13. Total length of inserts is four and five genome equivalents in the ICRF and LANL libraries, respectively. ICRF cosmids showed hybridization to 22 of 24 unique probes tested, which corresponds to a 90% probability of having any DNA fragment represented in the library. More than 1 Mb of chromosome 13 is overlapped by 90 cosmids of 22 groups revealed. A chromosomal region of more than 150 kb, containing the ATP1AL1 gene for alpha-1 peptide of Na+, K(+)-ATPase, is covered by 12 cosmids forming a contig. The results of restriction and hybridization analyses are stored in a CLONE database. These data and all the cosmids described are publicly available.
我们对分别由英国帝国癌症研究基金会（ICRF，13000个克隆）和美国洛斯阿拉莫斯国家实验室（LANL，17000个克隆）从经流式分选的13号染色体构建的两个黏粒文库进行了特征分析。储存两年后，克隆显示出高活力（95%）和结构稳定性。对500多个ICRF黏粒和200个LANL黏粒研究了EcoR I和Hind III酶切图谱。两个文库中插入片段的平均大小均显示为约35 - 37 kb。大多数黏粒（ICRF文库和LANL文库中分别为83%和93%）超过了可包装的DNA片段的下限大小，是13号染色体物理图谱构建的良好来源。ICRF文库和LANL文库中插入片段的总长度分别为四个和五个基因组当量。ICRF黏粒与所测试的24个独特探针中的22个发生杂交，这意味着文库中存在任何DNA片段的概率为90%。所揭示的22组中的90个黏粒覆盖了超过1 Mb的13号染色体区域。一个超过150 kb的染色体区域，包含Na⁺,K⁺-ATP酶α-1肽的ATP1AL1基因，被12个黏粒覆盖形成一个重叠群。酶切和杂交分析结果存储在一个CLONE数据库中。这些数据以及所描述的所有黏粒均可公开获取。
