Proshin S N, Kravtsov V Iu, Iakovlev A F, Kaminskaia E V, Vakhtin Iu B
Genetika. 1996 Mar;32(3):406-10.
Selection in vivo of cell clones of rat transplantable organospecific rhabdomyosarcoma RA-23 for increased and decreased frequencies of cells with chromosomal bridges (FCB) was performed. The initial average FCB in clones was 0.8% at a range of variation of from 0 to 3.0%. Selection for an increase, as well as for a decrease, in the FCB in RA-23 clones was effective. After one step of selection for an increase in the FCB, the average FCB increased to 3.0%. Thereafter, selection for an increase in the FCB was impracticable due to loss of transplantability in cell populations in which the FCB exceeded 5.0%. Over five steps of selection for a decrease in the FCB, the average FCB significantly decreased to 0.3% (P < 0.001). The heritability coefficient h2 of the trait FCB upon selection ranged from 0.25 to 0.30. The high and low FCBs attained by selection correlated with the index "frequency of cells with micronuclei." The population of RA-23 cells after selection for a decrease in the FCB differed from the population of cells of the original RA-23 strain by significantly lower karyotypic heterogeneity. The values of h2 obtained upon selection for the FCB and the effectiveness of selection for an increase, as well as for a decrease, in the FCB show that, with selection at the trait "frequency of cells with bridges," which characterizes the stability of the karyotype, existing cell strains can be subjected to karyotypic stabilization or destabilization.
对大鼠可移植器官特异性横纹肌肉瘤RA - 23的细胞克隆进行了体内选择，以增加和降低具有染色体桥的细胞频率（FCB）。克隆中初始平均FCB为0.8%，变化范围为0至3.0%。对RA - 23克隆中FCB的增加和减少进行选择均有效。在对FCB增加进行一步选择后，平均FCB增加到3.0%。此后，由于FCB超过5.0%的细胞群体失去移植性，对FCB增加的选择变得不可行。在对FCB减少进行五步选择后，平均FCB显著降低至0.3%（P < 0.001）。选择时性状FCB的遗传力系数h2在0.25至0.30之间。通过选择获得的高和低FCB与“微核细胞频率”指标相关。对FCB减少进行选择后的RA - 23细胞群体与原始RA - 23菌株的细胞群体相比，核型异质性显著降低。选择FCB时获得的h2值以及对FCB增加和减少选择的有效性表明，在以“有桥细胞频率”为特征的核型稳定性性状上进行选择时，现有细胞株可进行核型稳定或不稳定处理。
