Kravtsov V Iu, Kaminskaia E V, Iakovlev A F, Vakhtin Iu B
Tsitologiia. 1994;36(2):211-4.
Cell substrains with increased and decreased spontaneous levels of micronuclei have been obtained by artificial selection. Clones of the substrains were investigated for mitotic division fidelity. About 200 mitoses were investigated in each clone. Frequencies of mitotic abnormalities such as ana- and telophases with bridge, chromosome and fragment delays at ana- and telophases, scattered chromosomes, chromosomes dislocated from spindle at metaphases and tripolar ana- and telophases were significantly higher in substrains with increased level of micronuclei. This finding indicates that reasons for arising of spontaneous micronuclei and alterations in mitotic division fidelity are the same or closely related. These reasons can be amplified as a result of the artificial selection.
通过人工选择获得了微核自发水平升高和降低的细胞亚株。对这些亚株的克隆进行了有丝分裂保真度研究。每个克隆中研究了约200次有丝分裂。在微核水平升高的亚株中，有丝分裂异常的频率显著更高，如有桥的后期和末期、后期和末期的染色体和片段延迟、染色体分散、中期染色体从纺锤体脱位以及三极后期和末期。这一发现表明，自发微核产生的原因与有丝分裂保真度的改变相同或密切相关。这些原因可能会因人工选择而被放大。
