类固醇葡萄糖醛酸苷的放射免疫测定。作为半抗原的雌激素C-3葡萄糖醛酸苷。
The radioimmunoassay of steroid glucuronides. The oestrogen C-3 glucuronides as haptens.
作者信息
Samarajeewa P, Kellie A E
出版信息
Biochem J. 1975 Nov;151(2):369-76. doi: 10.1042/bj1510369.
Abstract
Antisera were prepared against three related oestrogen ring-A glucuronides, oestrone 3-glucuronide, oestradiol 3-glucuronide and oestriol 3-glucuronide. The corresponding 6,7-3H-labelled conjugates were synthesized as radioligands and the cross-reactions of the antisera against ring-A oestrogen glucuronides and other steroid conjugates were examined. The specificity of the antiserum against oestriol 3-glucuronide was compared with that raised against oestriol 16alpha-glucuronide, and the measurement of the former conjugate in late-pregnancy urine is discussed.
摘要
制备了针对三种相关雌激素A环葡糖醛酸苷、雌酮3 - 葡糖醛酸苷、雌二醇3 - 葡糖醛酸苷和雌三醇3 - 葡糖醛酸苷的抗血清。合成了相应的6,7 - 3H标记的结合物作为放射性配体，并检测了抗血清对A环雌激素葡糖醛酸苷和其他类固醇结合物的交叉反应。将抗雌三醇3 - 葡糖醛酸苷抗血清的特异性与抗雌三醇16α - 葡糖醛酸苷抗血清的特异性进行了比较，并讨论了在妊娠晚期尿液中对前一种结合物的测定。
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引用本文的文献
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The direct radioimmunoassay of oestrogen glucuronides in human female urine.人女性尿液中雌激素葡糖醛酸苷的直接放射免疫测定法。
Biochem J. 1979 Feb 1;177(2):729-38. doi: 10.1042/bj1770729.
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