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类固醇葡萄糖醛酸苷的放射免疫测定。作为半抗原的雌激素C-3葡萄糖醛酸苷。

The radioimmunoassay of steroid glucuronides. The oestrogen C-3 glucuronides as haptens.

作者信息

Samarajeewa P, Kellie A E

出版信息

Biochem J. 1975 Nov;151(2):369-76. doi: 10.1042/bj1510369.

DOI:10.1042/bj1510369
PMID:56173
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172367/
Abstract

Antisera were prepared against three related oestrogen ring-A glucuronides, oestrone 3-glucuronide, oestradiol 3-glucuronide and oestriol 3-glucuronide. The corresponding 6,7-3H-labelled conjugates were synthesized as radioligands and the cross-reactions of the antisera against ring-A oestrogen glucuronides and other steroid conjugates were examined. The specificity of the antiserum against oestriol 3-glucuronide was compared with that raised against oestriol 16alpha-glucuronide, and the measurement of the former conjugate in late-pregnancy urine is discussed.

摘要

制备了针对三种相关雌激素A环葡糖醛酸苷、雌酮3 - 葡糖醛酸苷、雌二醇3 - 葡糖醛酸苷和雌三醇3 - 葡糖醛酸苷的抗血清。合成了相应的6,7 - 3H标记的结合物作为放射性配体，并检测了抗血清对A环雌激素葡糖醛酸苷和其他类固醇结合物的交叉反应。将抗雌三醇3 - 葡糖醛酸苷抗血清的特异性与抗雌三醇16α - 葡糖醛酸苷抗血清的特异性进行了比较，并讨论了在妊娠晚期尿液中对前一种结合物的测定。

相似文献

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The radioimmunoassay of steroid glucuronides. The oestrogen C-3 glucuronides as haptens.类固醇葡萄糖醛酸苷的放射免疫测定。作为半抗原的雌激素C-3葡萄糖醛酸苷。
Biochem J. 1975 Nov;151(2):369-76. doi: 10.1042/bj1510369.
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The direct radioimmunoassay of oestrogen glucuronides in human female urine.人女性尿液中雌激素葡糖醛酸苷的直接放射免疫测定法。
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引用本文的文献

1
The direct radioimmunoassay of oestrogen glucuronides in human female urine.人女性尿液中雌激素葡糖醛酸苷的直接放射免疫测定法。
Biochem J. 1979 Feb 1;177(2):729-38. doi: 10.1042/bj1770729.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A study of the colorimetric estimation of oestradiol-17 beta, oestradiol-17 alpha, oestrone, oestriol and 16-epioestriol by the Kober reaction.一项关于通过科伯反应比色法测定17β-雌二醇、17α-雌二醇、雌酮、雌三醇和16-表雌三醇的研究。
Biochem J. 1961 Mar;78(3):593-602. doi: 10.1042/bj0780593.
3
Steroid-protein conjugates. II. Preparation and characterization of conjugates of bovine serum albumin with progesterone, deoxycorticosterone, and estrone.类固醇 - 蛋白质缀合物。II. 牛血清白蛋白与孕酮、脱氧皮质酮和雌酮缀合物的制备与表征。
J Biol Chem. 1959 May;234(5):1090-4.
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Steroid-protein conjugates. I. Preparation and characterization of conjugates of bovine serum albumin with testosterone and with cortisone.类固醇 - 蛋白质缀合物。I. 牛血清白蛋白与睾酮及可的松缀合物的制备与表征。
J Biol Chem. 1957 Oct;228(2):713-27.
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Comparative studies on hydrolysis of conjugated estrogens in human pregnancy urine.人妊娠尿液中结合雌激素水解的比较研究。
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The synthesis of oestrogen monoglucuronides.雌激素单葡萄糖醛酸苷的合成。
J Chem Soc Perkin 1. 1967;7:542-50. doi: 10.1039/j39670000542.
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Biosynthesis of radioactive estriol-3-glucosiduronate by guinea pig liver homogenate.
Steroids. 1965 Dec;6(6):859-64. doi: 10.1016/0039-128x(65)90106-6.
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The isolation of urinary 17-oxo steroid and corticosteroid glucuronides.尿中17-氧代类固醇和皮质类固醇葡糖醛酸苷的分离
Biochem J. 1964 May;91(2):209-17. doi: 10.1042/bj0910209.
9
Steroid conjugates. VI. An improved Koenigs-Knorr synthesis of aryl glucuronides using cadmium carbonate, a new and effective catalyst.
J Org Chem. 1971 Apr 9;36(7):863-70. doi: 10.1021/jo00806a001.
10
Steroid metabolism in the human foeto-placental unit.人类胎儿-胎盘单位中的类固醇代谢
Acta Endocrinol (Copenh). 1969 Aug;61(4):649-64. doi: 10.1530/acta.0.0610649.