al-Janadi N, al-Dalaan A, al-Balla S, Raziuddin S
Department of Medicine, College of Medicine, King Saud University, Abha, Saudi Arabia.
J Rheumatol. 1996 May;23(5):809-14.
Monocytes and CD4+/CD8+ T cells produce immunoregulatory cytokines that participate in the pathogenesis of various immune disorders. We investigated the secretion of Th1-Th2 cell response cytokine production of CD4+/CD8+ T cells from the synovial fluid (SF) and blood of patients with rheumatoid arthritis (RA).
Blood and SF purified monocytes, CD4+ and CD8+ T cells were stimulated with bacterial lipopolysaccharides or anti-CD3 antibody, and secretion of various cytokines was determined by bioassay or ELISA:
Monocytes from SF and blood of patients with RA produced highly elevated levels of interleukin-1 alpha (IL-1 alpha), IL-6, tumor necrosis factor-alpha (TNF-alpha), and granulocyte macrophage colony stimulating factor (GMCSF), the leading mediators of inflammation. However, CD4+ T cells secreted deficient levels of IL-2 and interferon-gamma (IFN-gamma), but higher levels of IL-4 and IL-10, the typical immunoregulatory Th2 cell response cytokines. CD8+ T cells also produce elevated levels of IL-4 and IL-10 but almost normal levels of IFN-gamma in this disease.
The cytokine produced by monocytes (IL-alpha, IL-6, TNF-alpha, and GMCSF) and by CD4+ T cells Th2 cell responses (IL-4 and IL-10) may exert immunopathologic and immunoregulatory effects in SF and thus mediate some of the clinical manifestations of RA.
单核细胞和CD4+/CD8+ T细胞产生免疫调节细胞因子，参与各种免疫紊乱的发病机制。我们研究了类风湿关节炎（RA）患者滑液（SF）和血液中CD4+/CD8+ T细胞的Th1-Th2细胞反应细胞因子的分泌情况。
用细菌脂多糖或抗CD3抗体刺激血液和SF纯化的单核细胞、CD4+和CD8+ T细胞，通过生物测定或ELISA法测定各种细胞因子的分泌情况。
RA患者SF和血液中的单核细胞产生高水平的白细胞介素-1α（IL-1α）、IL-6、肿瘤坏死因子-α（TNF-α）和粒细胞巨噬细胞集落刺激因子（GMCSF），这些是炎症的主要介质。然而，CD4+ T细胞分泌的IL-2和干扰素-γ（IFN-γ）水平不足，但IL-4和IL-10水平较高，这两种是典型的免疫调节Th2细胞反应细胞因子。在这种疾病中，CD8+ T细胞也产生高水平的IL-4和IL-10，但IFN-γ水平几乎正常。
单核细胞产生的细胞因子（IL-α、IL-6、TNF-α和GMCSF）以及CD4+ T细胞的Th2细胞反应（IL-4和IL-10）可能在SF中发挥免疫病理和免疫调节作用，从而介导RA的一些临床表现。
