Rab P, Reed K M, Ponce de León F A, Phillips R B
Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Academy of Sciences of Czech Republic.
Biotech Histochem. 1996 May;71(3):157-62. doi: 10.3109/10520299609117153.
A rapid method for detecting nucleolus organizer regions (NORs) in fish chromosomes based on thermal denaturation and staining with propidium iodide is described. Under epifluorescence, the NORs of 15 fish species from six families could be detected. This protocol differentiates constitutive heterochromatin in mammalian and avian chromosomes, and in some cases, heterochromatic blocks in fish chromosomes. The staining of NORs of fish chromosomes with propidium iodide following denaturation with formalin is likely the result of differential denaturation of the rDNA due to the thermal characteristics of AT- and GC-rich domains of the rDNA cistron. This technique provides a new useful marker for descriptive fish cytogenetic studies.
本文描述了一种基于热变性和碘化丙啶染色快速检测鱼类染色体核仁组织区（NORs）的方法。在落射荧光下，可检测六个科15种鱼类的NORs。该方法可区分哺乳动物和鸟类染色体中的组成型异染色质，在某些情况下，还可区分鱼类染色体中的异染色质区。用福尔马林变性后用碘化丙啶对鱼类染色体的NORs进行染色，可能是由于rDNA顺反子富含AT和GC区域的热特性导致rDNA的差异变性。该技术为鱼类描述性细胞遗传学研究提供了一种新的有用标记。
