Gartenhaus R, Hoffman M, Fuchs A, Billet H, Wang P, Gentile P, Rai K
Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, New York, USA.
Leuk Lymphoma. 1996 Jun;22(1-2):97-101, follow.186, color plate IX. doi: 10.3109/10428199609051734.
B-Chronic lymphocytic leukemia (B-CLL) and hairy cell leukemia (HCL) are both differentiated B-cell lymphoproliferative disorders. Prior studies have suggested that phorbol esters and the macrocyclic lactone Bryostatin-1, which are both protein kinase-C activators, can induce the differentiation of B-CLL cells into HCL cells in vitro, as evidenced by morphology, phenotype and TRAP activity. The differentiating effect of all-trans retinoic acid on B-CLL cells has been less extensively studied. We studied the effects of incubating adherence purified B-CLL cells with phorbol myristic acetate (PMA), all-trans retinoic acid (ATRA), and Bryostatin-1. None of these agents induced a true HCL phenotype (CD5-, CD11c/CD25 coexpression) under the conditions studied.
