al-Katib A, Wang C Y, McKenzie S, Clarkson B D, Koziner B
Department of Internal Medicine, Wayne State University, Detroit, Michigan 48202.
Am J Hematol. 1992 Aug;40(4):264-9. doi: 10.1002/ajh.2830400405.
Leukemic cells from eight patients with chronic lymphocytic leukemia were isolated and cultured in the continuous presence of 12-O-tetradecanoylphorbol 13-acetate (TPA) at a concentration of 1.6 x 10(-9) M for 4-10 days. Aliquots of cells were then analyzed at intervals of 24-72 hr for changes in morphology, acid phosphatase staining (AP), and expression of two hairy cell-associated surface antigens, HCL1 (CD22, Leu 14) and HCL3 (CD11c, Leu M5). All cases studied showed typical B-CLL phenotype, and only a small proportion of cells expressed CD22 and CD11c (mean 7% and 4.9%, respectively). TPA treatment induced the coexpression of CD22 (mean 49%) and CD11c (mean 48%) and tartrate-resistant acid phosphatase in seven of eight cases. Morphologically, cells in TPA cultures expressed hairy cell features that were evident in light and electron microscopic studies. Collectively these changes indicate that TPA can induce hairy cell features on CLL cells in vitro, suggesting the later maturational stage of HCL compared with CLL.
从8例慢性淋巴细胞白血病患者中分离出白血病细胞，并在12 - O - 十四烷酰佛波醇13 - 乙酸酯（TPA）浓度为1.6×10(-9) M持续存在的情况下培养4 - 10天。然后每隔24 - 72小时对细胞 aliquots 进行分析，观察形态学变化、酸性磷酸酶染色（AP）以及两种毛细胞相关表面抗原HCL1（CD22，Leu 14）和HCL3（CD11c，Leu M5）的表达情况。所有研究病例均表现出典型的B - CLL表型，只有一小部分细胞表达CD22和CD11c（分别平均为7%和4.9%）。TPA处理在8例中的7例中诱导了CD22（平均49%）和CD11c（平均48%）以及耐酒石酸酸性磷酸酶的共表达。形态学上，TPA培养的细胞在光学和电子显微镜研究中表现出明显的毛细胞特征。总体而言，这些变化表明TPA在体外可诱导CLL细胞出现毛细胞特征，提示与CLL相比，HCL处于更晚的成熟阶段。
