Berkova N, Lemay A, Boutin M, Turcot-Lemay L, Gagnon E
Laboratoire d'endocrinologie de la reproduction, Hôpital St-François d'Assise, Québec, Canada.
Hum Reprod. 1996 Apr;11(4):861-7.
Accumulated evidence implicates immunological alterations in endometriosis. The purpose of this study was to look for variations in antibodies to distinct antigens in peritoneal fluid of women with and without endometriosis. Peritoneal fluid was aspirated from 17 women undergoing laparoscopy for tubal ligation and 37 patients complaining of symptoms of pain and /or infertility. Peritoneal fluid antibodies to a standard preparation of peritoneal fluid antigens were detected by Western blot analysis using peroxidase-labelled anti-human immunoglobulin G antibodies specific to the Fc region. Antibodies to distinct antigens were quantified by estimating the ratio of the relative optical density between samples and a standard amount of antibodies. Marked changes were found in the antibody detection to two antigens having apparent molecular weights of 22 and 18 kDa. The intensity of the antibody signal was significantly weaker in the peritoneal fluid from endometriosis patients (0.36 +/- 0.06 and 0.46 +/- 0.06) compared with that in women without endometriosis (0.62 +/- 0.08 and 0.75 +/- 0.06). It was also weaker in patients without endometriosis presenting with infertility (0.36 +/- 0.07 and 0.47 +/- 0.08), but only the 18 kDa antigen result was significant. After adjusting for infertility, the P values for the 18 and 22 kDa bands were 0.03 and 0.28 (not significant) respectively in the group of endometriosis patients. These changes were not related to the phase of the menstrual cycle. These data suggest an alteration in the immune response to two distinct antigens in the peritoneal fluid from women with endometriosis and infertility. Further evaluation of these two antigens and their antibodies would be of interest to help understand endometriosis and its associated infertility.
越来越多的证据表明子宫内膜异位症存在免疫改变。本研究的目的是寻找患有和未患有子宫内膜异位症的女性腹膜液中针对不同抗原的抗体差异。从17名因输卵管结扎接受腹腔镜检查的女性和37名主诉疼痛和/或不孕症状的患者中抽取腹膜液。使用对Fc区域具有特异性的过氧化物酶标记抗人免疫球蛋白G抗体，通过蛋白质印迹分析检测腹膜液中针对腹膜液抗原标准制剂的抗体。通过估计样品与标准量抗体之间的相对光密度比值来定量针对不同抗原的抗体。在针对表观分子量为22 kDa和18 kDa的两种抗原的抗体检测中发现了显著变化。与未患子宫内膜异位症的女性（0.62±0.08和0.75±0.06）相比，子宫内膜异位症患者腹膜液中的抗体信号强度明显较弱（0.36±0.06和0.46±0.06）。在未患子宫内膜异位症但伴有不孕的患者中信号也较弱（0.36±0.07和0.47±0.08），但只有18 kDa抗原的结果具有统计学意义。在对不孕因素进行校正后，子宫内膜异位症患者组中18 kDa和22 kDa条带的P值分别为0.03和0.28（无统计学意义）。这些变化与月经周期阶段无关。这些数据表明患有子宫内膜异位症和不孕的女性腹膜液中对两种不同抗原的免疫反应发生了改变。对这两种抗原及其抗体进行进一步评估，将有助于理解子宫内膜异位症及其相关的不孕问题。
