乳头状膀胱尿路上皮癌中的嵌合BK病毒DNA附加体

Chimeric BK virus DNA episomes in a papillary urothelial bladder carcinoma.

作者信息

Monini P, de Lellis L, Rotola A, Di Luca D, Ravaioli T, Bigoni B, Cassai E

机构信息

Institute of Microbiology, University of Ferrara, Italy.

出版信息

Intervirology. 1995;38(5):304-8. doi: 10.1159/000150455.

DOI:10.1159/000150455

PMID:8724862

Abstract

BK virus (BKV) DNA sequences were identified in a papillary urothelial bladder carcinoma by Southern blot hybridization. The carcinoma contained both integrated and extrachromosomal DNA. Integrated sequences had a clonal restriction pattern, suggesting that BKV was integrated at some early stage of neoplastic initiation or progression. Viral episomes consisted of a population of covalent polymers based on a high-molecular-weight DNA unit, about 11-12 kb in size. DNA sequences non-homologous to the BKV genome were encompassed within DNA episomes, suggestive of acquisition of cellular sequences by viral DNA replication at the integration site. Extrachromosomal, chimeric DNA molecules were present at an average level of about 50 copies per cell, but their size, apparently incompatible with viral assembly, showed that BKV productive infection was impaired. The data suggest that infected cells underwent reversible changes affecting autonomous BKV DNA replication.

摘要

通过Southern印迹杂交在一例乳头状膀胱尿路上皮癌中鉴定出BK病毒（BKV）DNA序列。该癌组织中既含有整合型DNA，也含有染色体外DNA。整合序列具有克隆性限制性图谱，提示BKV在肿瘤起始或进展的早期阶段就已整合。病毒附加体由一群基于高分子量DNA单位的共价聚合物组成，大小约为11 - 12 kb。DNA附加体中包含与BKV基因组非同源的DNA序列，提示在整合位点病毒DNA复制过程中获取了细胞序列。染色体外嵌合DNA分子平均每个细胞约有50个拷贝，但它们的大小显然与病毒组装不兼容，表明BKV的有效感染受到损害。这些数据提示受感染细胞发生了影响BKV自主DNA复制的可逆性变化。