Shchelkunov S N, Totmenin A V, Sandakhchiev L S
Institute of Molecular Biology, State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk region, Russia.
Virus Res. 1996 Feb;40(2):169-83. doi: 10.1016/0168-1702(95)01269-9.
Sequencing and computer analysis of the nucleotide sequence of variola major virus strain India-1967 (VAR-IND) DNA segment (23 786 bp) covering the left variable region of the viral genome has been carried out. Twenty-nine potential open reading frames were identified. Structure-function organization of the VAR-IND DNA segment was compared with previously reported sequences from analogous genome regions of vaccinia virus strains Copenhagen (VAC-COP) and Western Reserve (VAC-WR). Multiple structural differences between the VAR-IND and genome regions were analysed and both VAC-COP and VAC-WR have been found. Possible molecular factors of virulence, virus host range genes as well as differences revealed in the structure of these genes of VAR and VAC will be discussed.
对天花病毒印度-1967株（VAR-IND）病毒基因组左可变区的DNA片段（23786 bp）进行了核苷酸序列测序和计算机分析。鉴定出29个潜在的开放阅读框。将VAR-IND DNA片段的结构-功能组织与先前报道的痘苗病毒哥本哈根株（VAC-COP）和西储株（VAC-WR）类似基因组区域的序列进行了比较。分析了VAR-IND与基因组区域之间的多个结构差异，并发现了VAC-COP和VAC-WR。将讨论可能的毒力分子因素、病毒宿主范围基因以及VAR和VAC这些基因结构中揭示的差异。
