Mercer A A, Fraser K M, Esposito J J
Health Research Council Virus Research Unit, University of Otago, Dunedin, New Zealand.
Virus Genes. 1996;13(2):175-8. doi: 10.1007/BF00568910.
About 47% identity was observed between the deduced amino acid sequences of a protein encoded by a gene of the parapoxvirus orf virus (OV) strain NZ2 and a 6 kDa protein of unknown function reported to be produced by an open reading frame expressed early after infection by the orthopoxvirus Western Reserve vaccinia virus (VAC); the open reading frame is absent from VAC strain Copenhagen. Examination of sequences reported for variola virus (VAR) strains Bangladesh, India, Congo- 1970, Somalia- 1977 and Garcia- 1966 revealed each encoded a correlate 58 amino acid protein. The open reading frame was not reported in the original analyses of these sequences because a lower limit of 60 amino acids was used to identify potential encoded proteins. Inspection of partial reading frames reported for cowpox virus (CWV) and ectromelia virus (EMV) suggested that these viruses might also code for a correlate of the VAC WR protein. DNA sequencing of cloned fragments of CWV and EMV confirmed that both these orthopoxviruses encode closely related, full length variants of the VAC and VAR open reading frames. The OV homologue is coded in the OV strain NZ2 BamHI-E fragment E2L open reading frame, which we reported is transcribed early postinfection; moreover, analysis of an NZ2 variant showed E2L was absent, indicating that E2L, like the VAC cognate, is nonessential for virus replication in cell culture. The parapoxvirus and orthopoxvirus correlates have about 20% amino acid sequence resemblance to African swine fever virus DNA binding protein p10, suggesting an ancestral relation of genes.
在副痘病毒羊口疮病毒（OV）株NZ2的一个基因编码的蛋白质推导氨基酸序列与据报道由正痘病毒西储痘苗病毒（VAC）感染后早期表达的一个开放阅读框产生的未知功能的6 kDa蛋白质之间，观察到约47%的同一性；VAC株哥本哈根中不存在该开放阅读框。对天花病毒（VAR）株孟加拉国、印度、刚果 - 1970、索马里 - 1977和加西亚 - 1966报道的序列进行检查发现，每个毒株都编码一种相关的58个氨基酸的蛋白质。在这些序列的原始分析中未报道该开放阅读框，因为使用了60个氨基酸的下限来鉴定潜在编码的蛋白质。对牛痘病毒（CWV）和鼠痘病毒（EMV）报道的部分阅读框的检查表明，这些病毒可能也编码与VAC WR蛋白相关的蛋白。CWV和EMV克隆片段的DNA测序证实，这两种正痘病毒都编码与VAC和VAR开放阅读框密切相关的全长变体。OV同源物编码在OV株NZ2 BamHI - E片段E2L开放阅读框中，我们报道该开放阅读框在感染后早期转录；此外，对NZ2变体的分析表明E2L缺失，这表明E2L与VAC同源物一样，对细胞培养中的病毒复制不是必需的。副痘病毒和正痘病毒的相关蛋白与非洲猪瘟病毒DNA结合蛋白p10有大约20%的氨基酸序列相似性，提示基因有祖先关系。
