MacCalman C D, Furth E E, Omigbodun A, Bronner M, Coutifaris C, Strauss J F
Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Dev Dyn. 1996 Jun;206(2):201-11. doi: 10.1002/(SICI)1097-0177(199606)206:2<201::AID-AJA9>3.0.CO;2-M.
Cadherin-11 is a novel member of the cadherin supergene family. Cadherin-11 expression is localized to mesenchymal tissue and specific regions of the neural tube during mouse embryogenesis. Here we report that cadherin-11 is spatiotemporally expressed in the epithelial cells of the human placenta. Cadherin-11 mRNA levels were low in freshly isolated cytotrophoblast cells but increased as the cytotrophoblast cells aggregated and fused to form syncytiotrophoblast cells in vitro. The increase in cadherin-11 mRNA levels was concomitant with a decrease in E-cadherin expression. Cadherin-11 was localized to the syncytial trophoblast and extravillous cytotrophoblasts, but not the villous cytotrophoblasts of the human placenta by immunohistochemistry. As both of the former cell types have intimate interactions with the endometrium, we examined cadherin-11 expression in the human endometrium. Cadherin-11 was detected in the glandular and surface epithelium of the endometrium at all stages of the menstrual cycle. However, cadherin-11 was abundant only in the stroma in the late secretory stage of the menstrual cycle. The accumulation of cadherin-11 in the stroma correlated with decidualization. Taken together, our observations demonstrate that cadherin-11 is expressed in certain epithelial cell lineages and suggest the possibility that cadherin-11 plays an important role in mediating trophoblast-endometrium interactions.
钙黏蛋白-11是钙黏蛋白超基因家族的一个新成员。在小鼠胚胎发育过程中，钙黏蛋白-11的表达定位于间充质组织和神经管的特定区域。在此我们报告，钙黏蛋白-11在人胎盘的上皮细胞中呈时空表达。在新鲜分离的细胞滋养层细胞中，钙黏蛋白-11的mRNA水平较低，但在体外细胞滋养层细胞聚集并融合形成合体滋养层细胞时其水平升高。钙黏蛋白-11 mRNA水平的升高与E-钙黏蛋白表达的降低同时发生。通过免疫组织化学方法发现，钙黏蛋白-11定位于人胎盘的合体滋养层和绒毛外细胞滋养层，而不是绒毛细胞滋养层。由于前两种细胞类型均与子宫内膜有密切相互作用，我们检测了人子宫内膜中钙黏蛋白-11的表达。在月经周期的所有阶段，均可在子宫内膜的腺上皮和表面上皮中检测到钙黏蛋白-11。然而，在月经周期的分泌晚期，钙黏蛋白-11仅在基质中大量存在。基质中钙黏蛋白-11的积累与蜕膜化相关。综上所述，我们的观察结果表明钙黏蛋白-11在某些上皮细胞谱系中表达，并提示钙黏蛋白-11在介导滋养层-子宫内膜相互作用中发挥重要作用的可能性。
