Tchernof A, Lamarche B, Prud'Homme D, Nadeau A, Moorjani S, Labrie F, Lupien P J, Després J P
Lipid Research Center, Laval University Medical (Research Center), Ste-Foy, Canada.
Diabetes Care. 1996 Jun;19(6):629-37. doi: 10.2337/diacare.19.6.629.
To investigate the potential relationship between the cluster of metabolic abnormalities found in visceral obesity and the small dense LDL phenotype.
We have estimated LDL peak particle size by nondenaturing 2-16% gradient gel electrophoresis in a sample of 79 men. Glucose tolerance and fasting plasma insulin and lipoprotein levels were also measured.
The LDL particle score, calculated from migration, distances and relative band intensities and reflecting the proportion of small dense LDL particles, was positively correlated with plasma triglyceride (TG) (r = 0.60, P < 0.0001) and negatively correlated with HDL cholesterol (r = -0.56, P < 0.0001) levels. Although the LDL particle score was not associated with variations in plasma LDL cholesterol or LDL apolipoprotein (apo) B concentrations, it was significantly correlated with the LDL apo B-to-LDL cholesterol ratio (r = 0.60, P < 0.0001). Fasting plasma insulin and visceral adipose tissue (AT) areas measured by computed tomography were weakly but significantly correlated with the LDL particle score (r = 0.23 and 0.29, respectively, P < 0.05). LDL peak particle size showed similar but inverse correlations with anthropometric and metabolic variables. Subjects classified as having small dense LDL particles (by comparing subjects in the highest tertile versus those in the lowest tertile of the LDL particle score distribution) were characterized by increased plasma TG, reduced HDL cholesterol, higher fasting insulin levels, and elevated visceral AT accumulation. However, multiple regression analyses revealed that visceral AT accumulation was not an independent predictor of the dense LDL phenotype after inclusion of TG and HDL cholesterol levels and lipoprotein ratios in the model.
It thus appears that the high TG-low HDL cholesterol dyslipidemia frequently found in visceral obesity and in a hyperinsulinemic state is a strong correlate of the small dense LDL phenotype. Although associated with the dense LDL phenotype, visceral obesity and hyperinsulinemia were not independent predictors of an increased proportion of small dense LDL particles after controlling for TG and HDL cholesterol levels.
研究内脏性肥胖中发现的代谢异常簇与小而密低密度脂蛋白（LDL）表型之间的潜在关系。
我们通过非变性2 - 16%梯度凝胶电泳，在79名男性样本中估计了LDL峰值颗粒大小。还测量了葡萄糖耐量、空腹血浆胰岛素和脂蛋白水平。
根据迁移距离和相对条带强度计算得出的LDL颗粒评分反映了小而密LDL颗粒的比例，与血浆甘油三酯（TG）呈正相关（r = 0.60，P < 0.0001），与高密度脂蛋白胆固醇呈负相关（r = -0.56，P < 0.0001）。虽然LDL颗粒评分与血浆LDL胆固醇或LDL载脂蛋白（apo）B浓度的变化无关，但它与LDL apo B与LDL胆固醇的比值显著相关（r = 0.60，P < 0.0001）。空腹血浆胰岛素和通过计算机断层扫描测量的内脏脂肪组织（AT）面积与LDL颗粒评分呈弱但显著的相关（分别为r = 0.23和0.29，P < 0.05）。LDL峰值颗粒大小与人体测量和代谢变量呈现相似但相反的相关性。被归类为具有小而密LDL颗粒的受试者（通过比较LDL颗粒评分分布最高三分位数与最低三分位数的受试者）的特征是血浆TG升高、高密度脂蛋白胆固醇降低、空腹胰岛素水平升高以及内脏AT积累增加。然而，多元回归分析显示，在模型中纳入TG和高密度脂蛋白胆固醇水平及脂蛋白比值后，内脏AT积累并非致密LDL表型的独立预测因素。
因此，在内脏性肥胖和高胰岛素血症状态中常见的高TG - 低高密度脂蛋白胆固醇血脂异常似乎与小而密LDL表型密切相关。虽然与致密LDL表型相关，但在控制TG和高密度脂蛋白胆固醇水平后，内脏性肥胖和高胰岛素血症并非小而密LDL颗粒比例增加的独立预测因素。
